Li Liqin, Deng Mengsheng, Lyu Chengcheng, Zhang Jie, Peng Jie, Cai Chengcheng, Yang Shimin, Lu Liming, Ni Su, Liu Fan, Zheng Shunlin, Yu Liping, Wang Xiyao
College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China.
Food Chem. 2020 Apr 21;325:126875. doi: 10.1016/j.foodchem.2020.126875.
Brassinosteroids (BRs), a new class of steroid hormones, are involved in the regulation of plant cell elongation and seed germination. Nevertheless, the molecular mechanism of the effect of BRs on tuber sprouting remains largely unknown. In this study, quantitative phosphoproteomics was employed to investigate the protein phosphorylation changes in sprouting induced by BRs. Our results showed that BRs accelerated the conversion of starch into soluble sugar in tubers. A functional enrichment cluster analysis suggested that the "amino acid metabolism pathway" was upregulated and that "plant hormone signal transduction and protein export" were downregulated. BR treatment also changed the phosphorylation of proteins involved in the BR, ABA, starch and sugar signal transduction pathways, such as serine/threonine-protein kinase (BSK), 14-3-3, alpha-glucan water dikinase (GWD), sucrose-phosphate synthase (SPS), sucrose synthase (SS) and alkaline/neutral invertase (A/N-INV). These results shed more light on the pattern of protein phosphorylation in BR promoting potato sprouting.
油菜素甾醇（BRs）是一类新型甾体激素，参与植物细胞伸长和种子萌发的调控。然而，BRs对块茎发芽影响的分子机制仍 largely未知。在本研究中，采用定量磷酸化蛋白质组学来研究BRs诱导发芽过程中的蛋白质磷酸化变化。我们的结果表明，BRs加速了块茎中淀粉向可溶性糖的转化。功能富集聚类分析表明，“氨基酸代谢途径”上调，“植物激素信号转导和蛋白质输出”下调。BR处理还改变了参与BR、ABA、淀粉和糖信号转导途径的蛋白质的磷酸化，如丝氨酸/苏氨酸蛋白激酶（BSK）、14-3-3、α-葡聚糖水二激酶（GWD）、蔗糖磷酸合酶（SPS）、蔗糖合酶（SS）和碱性/中性转化酶（A/N-INV）。这些结果为BR促进马铃薯发芽过程中的蛋白质磷酸化模式提供了更多线索。
