The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, China.
Key Laboratory of Oral Diseases Research of Anhui Province, Stomatological Hospital & College, Anhui Medical University, Hefei, China.
Oral Dis. 2021 Jul;27(5):1073-1089. doi: 10.1111/odi.13405. Epub 2020 May 26.
This systematic review was to evaluate the change of oral microbiome based on next-generation sequencing (NGS)-metagenomic analysis following periodontal interventions among systematically healthy subjects.
A structured search strategy consisting of "metagenomics" and "oral diseases" was applied to PubMed, EMBASE, and Web of Science to identify effective papers. The included studies were original studies published in English, using metagenomic approach to analyze the effectiveness of periodontal intervention on oral microbiome among systematically healthy human subjects with periodontitis.
A total of 12 papers were included in this review. Due to the heterogeneity of selected study, quantitative analysis was not performed. The findings as to how alpha diversity changed after interventions were not consistent across studies. Six studies illustrated clear separation of microbial composition between dental plaque samples collected before and after intervention using principal coordinates/component analysis. The most commonly detected genera before intervention were Porphyromonas, Treponema, Tannerella, and Prevotella, while Streptococcus and Actinomyces usually increased and became the dominant genera after intervention. Correlation network analysis revealed that after intervention, the topology of network was different compared to the corresponding pre-interventional samples.
Existing evidence of metagenomic studies depicts a complex change in oral microbiome after periodontal intervention.
本系统评价通过下一代测序（NGS）-宏基因组分析，评估牙周干预后系统性健康受试者口腔微生物组的变化。
采用“宏基因组学”和“口腔疾病”的结构化检索策略，在 PubMed、EMBASE 和 Web of Science 中检索有效文献。纳入的研究为使用宏基因组方法分析牙周炎系统性健康受试者牙周干预对口腔微生物组影响的原始英文研究。
本综述共纳入 12 篇文献。由于所选研究存在异质性，未进行定量分析。干预后 alpha 多样性变化的结果在不同研究中并不一致。六项研究表明，使用主坐标/成分分析，干预前后牙菌斑样本的微生物组成明显分离。干预前最常见的检测到的属为卟啉单胞菌、密螺旋体、丹毒丝菌和普雷沃菌，而链球菌和放线菌通常在干预后增加并成为优势属。相关网络分析显示，干预后，网络的拓扑结构与相应的干预前样本不同。
宏基因组研究的现有证据表明，牙周干预后口腔微生物组发生了复杂的变化。
