Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, P. R. China.
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, P. R. China.
Mol Plant Microbe Interact. 2020 Aug;33(8):1080-1090. doi: 10.1094/MPMI-11-19-0325-R. Epub 2020 Jun 29.
, the causative pathogen of Marssonina leaf spot of poplars (MLSP), devastates poplar plantations by forming black spots on leaves and defoliating trees. Although MLSP has been studied for over 30 years, the key genes that function during infection and their effects on plant growth are poorly understood. Here, we used multigene association studies to investigate the effects of key genes in the plant-pathogen interaction pathway, as revealed by transcriptome analysis, on photosynthesis and growth in a natural population of 435 individuals. By analyzing transcriptomic changes during three stages of infection, we detected 628 transcription factor genes among the 7,611 differentially expressed genes that might be associated with basal defense responses. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that transcriptomic changes across different stages of infection lead to the reprogramming of metabolic processes possibly related to defense activation. We identified 29,399 common single-nucleotide polymorphisms (SNPs) within 221 full-length genes in plant-pathogen interaction pathways that were significantly associated with photosynthetic and growth traits. We also detected 4,460 significant epistatic pairs associated with stomatal conductance, tree diameter, and tree height. Epistasis analysis uncovered significant interactions between 2,561 SNP-SNP pairs from different functional modules in the plant-pathogen interaction pathway, revealing possible genetic interactions. This analysis revealed many key genes that function during infection and their potential roles in mediating photosynthesis and plant growth, shedding light on genetic interactions between functional modules in the plant-pathogen interaction pathway.
杨树球腔菌（Marssonina leaf spot of poplars，MLSP）是导致杨树叶斑病的病原菌，它会在叶片上形成黑斑并导致树木落叶，从而严重破坏人工杨树林。尽管对 MLSP 的研究已经超过 30 年，但对于感染过程中起作用的关键基因及其对植物生长的影响仍知之甚少。在这里，我们使用多基因关联研究来调查转录组分析揭示的植物-病原体互作途径中的关键基因对自然种群中 435 个个体光合作用和生长的影响。通过分析感染三个阶段的转录组变化，我们在 7611 个差异表达基因中检测到 628 个转录因子基因，这些基因可能与基础防御反应有关。基因本体论和京都基因与基因组百科全书富集分析表明，不同感染阶段的转录组变化导致代谢过程的重新编程，这可能与防御激活有关。我们在植物-病原体互作途径中的 221 个全长基因中鉴定出 29399 个与光合作用和生长性状显著相关的常见单核苷酸多态性（SNP）。我们还检测到 4460 个与气孔导度、树径和树高显著相关的上位性对。上位性分析揭示了植物-病原体互作途径中不同功能模块的 2561 个 SNP-SNP 对之间存在显著的相互作用，揭示了可能的遗传相互作用。这项分析揭示了许多在感染过程中起作用的关键基因及其在介导光合作用和植物生长中的潜在作用，阐明了植物-病原体互作途径中功能模块之间的遗传相互作用。
