Tsukatani Tadayuki, Sakata Fumihiko, Kuroda Rieko, Akao Tetsuyuki
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawamachi, Kurume, 839-0861, Japan.
Fukuoka Industrial Technology Center, Chikushino, Japan.
Curr Microbiol. 2020 Sep;77(9):2486-2495. doi: 10.1007/s00284-020-02017-z. Epub 2020 May 11.
The purpose of this study was to select herbs and spices with potent biofilm eradication activities. Further, the combined effects of herb and spice extracts against pathogenic biofilms were evaluated. The biofilm eradication activities of ethanol extracts of 104 herbs and spices were measured by combining a colorimetric microbial viability assay with a biofilm formation technique. Ethanol extract of clove had potent biofilm eradication activities against Escherichia coli, Porphyromonas gingivalis, and Streptococcus mutans. Ethanol extracts of eucalyptus and rosemary had potent biofilm eradication activities against P. gingivalis, Staphylococcus aureus and S. mutans. The combination of extracts of clove with eucalyptus or rosemary showed synergistic or additive effects, or both, on biofilm eradication activities. The main biofilm inhibitors in the ethanol extracts of clove, eucalyptus and rosemary were eugenol, macrocarpals and carnosic acid, respectively. The combinations of extracts of clove with eucalyptus or rosemary had potent biofilm eradication activities against oral and food-borne pathogenic bacteria. The findings of the present study reveal that specific combinations of herb and spice extracts may prevent and control biofilm-related oral diseases, food spoilage, and food poisoning.
本研究的目的是筛选具有强效生物膜清除活性的药草和香料。此外，还评估了药草和香料提取物对致病性生物膜的联合作用。通过将比色法微生物活力测定与生物膜形成技术相结合，测定了104种药草和香料乙醇提取物的生物膜清除活性。丁香乙醇提取物对大肠杆菌、牙龈卟啉单胞菌和变形链球菌具有强效生物膜清除活性。桉叶和迷迭香乙醇提取物对牙龈卟啉单胞菌、金黄色葡萄球菌和变形链球菌具有强效生物膜清除活性。丁香提取物与桉叶或迷迭香提取物的组合在生物膜清除活性上表现出协同或相加作用，或两者兼具。丁香、桉叶和迷迭香乙醇提取物中的主要生物膜抑制剂分别为丁香酚、大果桉醇和鼠尾草酸。丁香提取物与桉叶或迷迭香提取物的组合对口腔和食源性病原体具有强效生物膜清除活性。本研究结果表明，药草和香料提取物的特定组合可能预防和控制与生物膜相关的口腔疾病、食物变质和食物中毒。
