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脉冲电场提取对 L. 叶提取物抗氧化、抗酪氨酸酶和透明质酸酶活性的增强作用。

Enhancement of the Antioxidant, Anti-Tyrosinase, and Anti-Hyaluronidase Activity of L. Leaf Extract by Pulsed Electric Field Extraction.

机构信息

Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.

Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand.

出版信息

Molecules. 2020 May 8;25(9):2212. doi: 10.3390/molecules25092212.

DOI:10.3390/molecules25092212
PMID:32397313
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7249078/
Abstract

In this study we aimed to compare the chemical composition and biological activity between L. leaf extract obtained with 95% / ethanol using a pulsed electric field (PEF) and the conventional maceration method. Extracts of leaves collected from Chiang Mai (CM), Sakon Nakon (SK), and Buriram (BR), Thailand, were investigated for 1-deoxynojirimycin content by high-performance liquid chromatography and for total phenolic content by the Folin-Ciocalteu method. Antioxidant activity was investigated by 2,2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS), and ferric reducing antioxidant power (FRAP) assay. Anti-tyrosinase and anti-hyaluronidase activity was investigated by in vitro spectrophotometry. The results show that this is the first study to indicate PEF as a novel method for enhancing the phenolic content and antioxidant, anti-tyrosinase, and anti-hyaluronidase activity of leaf extract ( < 0.05). PEF extract of leaves collected from BR had comparable ABTS scavenging activity to l-ascorbic acid and comparable anti-tyrosinase activity to kojic acid ( > 0.05). On the other hand, PEF extract of leaves collected from SK exhibited significantly high anti-hyaluronidase activity, comparable to that of oleanolic acid ( > 0.05). Therefore, PEF is suggested for further leaf extraction in the production of natural whitening and anti-aging cosmetic ingredients.

摘要

在这项研究中，我们旨在比较使用脉冲电场（PEF）从 95%乙醇中获得的 L.叶提取物与传统浸渍法之间的化学成分和生物活性。我们对来自泰国清迈（CM）、色军（SK）和武里南（BR）的 叶提取物进行了 1-脱氧野尻霉素含量的高效液相色谱分析和总酚含量的福林-酚比色法分析。抗氧化活性通过 2,2'-二苯基-1-苦基肼基（DPPH）、2,2'-联氮-3-乙基苯并噻唑啉-6-磺酸（ABTS）和铁还原抗氧化能力（FRAP）测定法进行研究。通过体外分光光度法研究了对酪氨酸酶和透明质酸酶的抑制活性。结果表明，这是首次表明 PEF 可作为一种增强 叶提取物的酚含量和抗氧化、抗酪氨酸酶和抗透明质酸酶活性的新方法（<0.05）。BR 采集的 叶的 PEF 提取物具有与 l-抗坏血酸相当的 ABTS 清除活性，且具有与曲酸相当的抗酪氨酸酶活性（>0.05）。另一方面，SK 采集的 叶的 PEF 提取物表现出显著高的透明质酸酶抑制活性，与齐墩果酸相当（>0.05）。因此，建议在生产天然美白和抗衰老化妆品成分时进一步使用 PEF 进行 叶提取。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/53da924c226f/molecules-25-02212-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/e74a8b54b1bc/molecules-25-02212-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/426917ab1d3d/molecules-25-02212-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/c5c9d9d4d4e9/molecules-25-02212-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/7922f3f70e87/molecules-25-02212-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/8d907e053660/molecules-25-02212-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/de8e1537ebbb/molecules-25-02212-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/53da924c226f/molecules-25-02212-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/e74a8b54b1bc/molecules-25-02212-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/426917ab1d3d/molecules-25-02212-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/c5c9d9d4d4e9/molecules-25-02212-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/7922f3f70e87/molecules-25-02212-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/8d907e053660/molecules-25-02212-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/de8e1537ebbb/molecules-25-02212-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcc5/7249078/53da924c226f/molecules-25-02212-g007.jpg

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