Laboratory of Biochemistry, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Laboratory of Biochemistry, Department of Biochemistry, Faculty of Science, University of Bamenda, Bambili, Cameroon.
J Med Food. 2021 Feb;24(2):180-187. doi: 10.1089/jmf.2020.0005. Epub 2020 May 12.
Mushroom contains a wide variety of components with high immune-modulating properties. Mushroom polysaccharides are known as immunocorrectors with wide areas of usage, which balance the immune system without resulting in the entire immune suppression or overreaction. To explore the potentially immunomodulatory role of , the present study was intended to evaluate the immunomodulatory activity of polysaccharides (CPP) on rat neutrophils and macrophages. CPP was studied on lipopolysaccharide-induced neutrophils and macrophages' responses. The effect on phagocytosis, release of reactive oxygen intermediates (ROI), nitric oxide (NO), lysosomal enzyme release and neutrophil myeloperoxidase, and neutrophil adherence was assessed. The cytotoxicity of CPP against macrophages using 3-(4,5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide assay and neutral red inclusion assay was also assessed. ENPC (endopolysaccharides) markedly enhanced phagocytosis of macrophages and neutrophils, whereas the EXPC (exopolysaccharides) increased only that of neutrophils at 0.34 and 0.68 mg/mL. The production of NO evidently increased in ENPC- and EXPC-treated neutrophil and macrophage cultures. The production of ROI in a concentration-dependent manner increased in ENPC-treated (0.08-5.55 mg/mL) and EXPC-treated (0.17 and 0.34 mg/mL) macrophage cultures and ENPC-treated neutrophils, whereas it decreased in EXPC-treated neutrophils. The secretion of acid phosphatase increased in ENPC- and EXPC-treated macrophage and neutrophil cultures. Neutrophil myeloperoxidase release increased in EXPC-treated (5.55 mg/mL) cultures, whereas it decreased in ENPC-treated cultures. ENPC and EXPC increased the adhesion of neutrophils to microplate. These findings demonstrated that CPP displayed a dual mode of action, inhibiting or enhancing numerous phagocytic activities of macrophages and neutrophils.
蘑菇含有多种具有高度免疫调节特性的成分。蘑菇多糖被称为免疫调节剂，具有广泛的用途，它可以平衡免疫系统，而不会导致整个免疫系统受到抑制或过度反应。为了探索 的潜在免疫调节作用，本研究旨在评估 多糖（CPP）对大鼠中性粒细胞和巨噬细胞的免疫调节活性。CPP 研究了脂多糖诱导的中性粒细胞和巨噬细胞的反应。评估了吞噬作用、活性氧中间体（ROI）、一氧化氮（NO）、溶酶体酶释放和中性粒细胞髓过氧化物酶以及中性粒细胞黏附的变化。使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定法和中性红包容测定法评估 CPP 对巨噬细胞的细胞毒性。ENPC（内多糖）显著增强了巨噬细胞和中性粒细胞的吞噬作用，而 EXPC（外多糖）仅在 0.34 和 0.68mg/mL 时增加了中性粒细胞的吞噬作用。NO 的产生在 ENPC 和 EXPC 处理的中性粒细胞和巨噬细胞培养物中明显增加。ROI 的产生以浓度依赖的方式增加，在 ENPC 处理（0.08-5.55mg/mL）和 EXPC 处理（0.17 和 0.34mg/mL）的巨噬细胞培养物和 ENPC 处理的中性粒细胞中增加，而在 EXPC 处理的中性粒细胞中减少。酸性磷酸酶的分泌在 ENPC 和 EXPC 处理的巨噬细胞和中性粒细胞培养物中增加。EXPC 处理（5.55mg/mL）的培养物中中性粒细胞髓过氧化物酶的释放增加，而 ENPC 处理的培养物中则减少。ENPC 和 EXPC 增加了中性粒细胞对微孔板的黏附。这些发现表明 CPP 表现出双重作用模式，抑制或增强巨噬细胞和中性粒细胞的多种吞噬活性。
