地克珠利纳米乳剂控制肉鸡柔嫩艾美耳球虫的效果与安全性。
Efficacy and safety of diclazuril nanoemulsion in control of Eimeria tenella in broilers.
机构信息
Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
出版信息
BMC Vet Res. 2024 Oct 29;20(1):495. doi: 10.1186/s12917-024-04325-z.
BACKGROUND
Nanotechnology has the potential to reduce drug dosage while increasing efficacy; thus, the current work intends to synthesize diclazuril nanoemulsion and assess its performance against experimental coccidiosis in broilers.
METHODS
Diclazuril nanoemulsion (DZN) was formulated and characterized by zeta seizer and zeta potential. The formulated DZN was evaluated in vivo against Eimeria tenella infected chicks. DZN and DZ were used in 2 programs; therapeutic and prophylactic. A total of 210 one-day-old broiler chicks were distributed equally into six groups. The controls were negative uninfected untreated and positive infected untreated (G1 & G2). Therapeutic groups (G3 & G4) treated by DZ and DZN after appearance of the clinical signs of coccidiosis and continued for 5 days. Prophylaxis groups (G5 & G6) received DZ and DZN at 3 days before challenge and continued for 5 days after infection. The treatments dosages were 10 mg/mL for DZ of commercial origin and 2.5 mg/mL for the prepared DZN. All groups (except negative control) orally infected then followed up for clinical signs of coccidiosis, mortality rate, oocysts count, performance, hematological and biochemical parameters in addition to histopathological lesions.
RESULTS
The therapeutic groups showed that both treated groups (DZ and DZN) revealed similar results including good body weight gain, a low lesion caecal score, a low daily and total oocyst shedding count, and a low mortality rate. Regarding the biochemical parameters, all parameters were affected during infection then restored after the 12th day post infection. However, in the prophylactic groups, showed mild clinical signs and the blood pictures and biochemical parameters were nearly like the control negative without infection.
CONCLUSION
DZN at a quarter dose of standard DZ produced the same outcomes as DZ at 10 mg/mL. Furthermore, DZN does not impair the typical safety of diclazuril in treated chicks.
背景
纳米技术有可能降低药物剂量，同时提高疗效；因此，目前的工作旨在合成地克珠利纳米乳剂，并评估其对肉鸡球虫病的疗效。
方法
用纳米粒度仪和动电位仪对地克珠利纳米乳剂（DZN）进行配方和特性描述。用体内试验方法评估所制备的 DZN 对柔嫩艾美耳球虫感染小鸡的作用。DZN 和 DZ 用于两种方案；治疗和预防。将 210 只 1 日龄肉鸡雏鸡平均分为 6 组。对照组为阴性、未感染、未治疗组（G1 和 G2）。治疗组（G3 和 G4）在出现球虫病临床症状后，用 DZ 和 DZN 治疗，持续 5 天。预防组（G5 和 G6）在攻毒前 3 天用 DZ 和 DZN 给药，感染后继续给药 5 天。DZ 的治疗剂量为 10mg/mL，市售 DZ 为 2.5mg/mL。所有组（阴性对照组除外）均口服感染，然后观察球虫病临床症状、死亡率、卵囊数、生产性能、血液学和血液生化参数以及组织病理学病变。
结果
治疗组结果表明，两组治疗组（DZ 和 DZN）的结果相似，包括体重增加良好、盲肠病变评分低、日总卵囊脱落计数低、死亡率低。关于血液生化参数，所有参数在感染期间均受到影响，感染后第 12 天恢复正常。然而，在预防组中，临床症状轻微，血液学和血液生化参数与未感染的阴性对照组相似。
结论
DZN 的剂量为标准 DZ 的四分之一，但产生的效果与 10mg/mL 的 DZ 相同。此外，DZN 不会损害治疗鸡对地克珠利的典型安全性。
Zotero 插件