Saber Alshimaa, Mawas Amany Sayed, Khalil Atef M, Ahmed Ahmed I, Shibat Elhamd Dina M W, Ali Esraa, Rehan Ibrahim F, Elnagar Asmaa, Zigo František, Salah Mohammed
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Division of Pathology and Clinical Pathology, Agricultural Research Centre (ARC), Animal Health Research Institute (AHRI), Qena, Egypt.
Front Vet Sci. 2025 Mar 31;12:1511759. doi: 10.3389/fvets.2025.1511759. eCollection 2025.
Coccidiosis is a significant disease with economic implications. It causes high mortality and morbidity, often associated with weight loss. This study investigated the effects of ginger supplementation on antioxidant status, biochemical parameters, and intestinal tissue histopathology in experimentally induced coccidiosis. One hundred one-day-old broiler chicks (Ross) were purchased from a local hatchery. Birds were kept in a clean, well ventilated and disinfected shed. The birds were given non-pelleted diets and water ad libitum throughout the period of the study (25 days). The aqueous ginger was administered orally to the chicks of the four dietary groups via drinking water at concentrations of 6 gm/L of water. All biosecurity measures were adopted according to standard protocol. The chicks were allocated into five groups: control negative (CN), control positive (CP) was infected with 3 × 10 sporulated oocysts at day 14th of the experiment, the third group (GO) was given ginger only for 25 days, the fourth group (GB) was given ginger from day 4th to day 25th of the experiment, and the fifth group (GA) was given ginger from day 5th post infection to day 25th of the experiment. Ginger extract treatment reduced the fecal oocyst count in the infected group, particularly on day 10 post-infection. The alterations in differential leukocyte counts due to coccidial infection were ameliorated by ginger extract administration. Biochemically, compared to the infected group, ginger () reversed the altered biochemical parameters (total protein, albumin, MDA, and SOD enzyme) associated with cecal coccidiosis. Immunologically, ginger extract treatment increased CD4 T cell counts and overexpression of INF- in the cecal epithelium. Histological examination revealed a significant reduction in goblet cell number and a decrease in the villus height (VH) to crypt depth (CD) ratio in infected chicks. Restoration of normal cecal histological structure and increased absorptive function and goblet cell number were observed in ginger-treated chicks compared to control-positive chicks. In conclusion, ginger supplements have effective therapeutic uses against intestinal coccidiosis as shown in the biochemical, immunohistochemical and histopathological results.
球虫病是一种具有经济影响的重要疾病。它会导致高死亡率和高发病率，常伴有体重减轻。本研究调查了补充生姜对实验性诱导的球虫病中抗氧化状态、生化参数和肠道组织组织病理学的影响。从当地孵化场购买了100只1日龄的肉鸡雏鸡（罗斯品种）。将鸡饲养在干净、通风良好且经过消毒的鸡舍中。在整个研究期间（25天），给鸡提供非颗粒饲料和自由饮水。通过饮用水以6克/升水的浓度将生姜水口服给予四个饮食组的雏鸡。所有生物安全措施均按照标准方案采用。将雏鸡分为五组：阴性对照组（CN）、阳性对照组（CP）在实验第14天感染3×10个孢子化卵囊，第三组（GO）仅在25天内给予生姜，第四组（GB）在实验第4天至第25天给予生姜，第五组（GA）在感染后第5天至实验第25天给予生姜。生姜提取物处理降低了感染组的粪便卵囊计数，尤其是在感染后第10天。生姜提取物的施用改善了球虫感染引起的白细胞分类计数变化。在生化方面，与感染组相比，生姜（此处原文似乎不完整）逆转了与盲肠球虫病相关的生化参数（总蛋白、白蛋白、丙二醛和超氧化物歧化酶）的改变。在免疫方面，生姜提取物处理增加了盲肠上皮中CD(_4) T细胞计数和INF的过表达。组织学检查显示，感染雏鸡的杯状细胞数量显著减少，绒毛高度（VH）与隐窝深度（CD）之比降低。与阳性对照雏鸡相比，在生姜处理的雏鸡中观察到盲肠组织结构恢复正常，吸收功能增强，杯状细胞数量增加。总之，生化、免疫组化和组织病理学结果表明，补充生姜对肠道球虫病具有有效的治疗作用。
