Cimmino Alessio, Roscetto Emanuela, Masi Marco, Tuzi Angela, Radjai Imene, Gahdab Chakali, Paolillo Rossella, Guarino Amedeo, Catania Maria Rosaria, Evidente Antonio
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, via Cintia 4, 80126 Napoli, Italy.
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via Pansini 5, 80131 Napoli, Italy.
Antibiotics (Basel). 2021 Jul 6;10(7):819. doi: 10.3390/antibiotics10070819.
, belonging to the tribe Anthemideae, is a plant widespread in the Southern hemisphere. It is frequently used in folk medicine in North African countries for several of its medical properties, shown by its extracts and essential oils. The dichloromethane extract obtained from its aerial parts demonstrated antibiotic activity against and was fractionated by bioguided purification procedures affording five main sesquiterpene lactones. They were identified by spectroscopic methods (NMR and ESIMS data) as guaiantrienolides, i.e., 6-acetoxy-1β-,6-acetoxy-1α-, and 6-acetoxy-10-β-hydroxyguaiantrienolide (-), and germacrenolides, i.e., haagenolide and 1,10-epoxyhaagenolide ( and ). The absolute configuration was assigned by applying the advanced Mosher's method to haagenolide and by X-ray diffraction analysis to 1,10-epoxyhaagenolide. The specific antibiotic and antibiofilm activities were tested toward the clinical isolates of . The results showed that compounds - have antibacterial activity against all the strains of , while compound exhibited activity only toward some strains. Compound did not show this activity but had only antibiofilm properties. Thus, these metabolites have potential as new antibiotics and antibiofilm against drug resistant opportunistic pathogens.
属于春黄菊族，是一种广泛分布于南半球的植物。由于其提取物和精油所显示的多种药用特性，它在北非国家的民间医学中经常被使用。从其地上部分获得的二氯甲烷提取物对……显示出抗生素活性，并通过生物导向纯化程序进行分馏，得到五种主要的倍半萜内酯。通过光谱方法（核磁共振和电喷雾离子化质谱数据）将它们鉴定为愈创木三烯醇内酯，即6 - 乙酰氧基 - 1β -、6 - 乙酰氧基 - 1α - 和6 - 乙酰氧基 - 10 - β - 羟基愈创木三烯醇内酯（ - ），以及吉马烯醇内酯，即哈根诺内酯和1,10 - 环氧哈根诺内酯（ 和 ）。通过对哈根诺内酯应用先进的莫舍尔方法以及对1,10 - 环氧哈根诺内酯进行X射线衍射分析来确定绝对构型。针对……的临床分离株测试了其特定的抗生素和抗生物膜活性。结果表明，化合物 - 对所有……菌株均具有抗菌活性，而化合物 仅对某些菌株表现出活性。化合物 未显示出这种活性，但仅具有抗生物膜特性。因此，这些代谢产物作为针对耐药性机会性病原体的新型抗生素和抗生物膜剂具有潜力。
