Departments of Ethnopharmacology and Pharmacognosy, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, 52000, Errachidia, Morocco.
Endocr Metab Immune Disord Drug Targets. 2021;21(6):1043-1052. doi: 10.2174/1871530320666200929141140.
The aim of the study was to assess the antihyperglycemic activity of Brassica oleracea.
Collard green or Brassica oleracea var. viridis which belongs to Brassicaceae family is ranked at 10th place of "powerhouse" vegetables, and it is commonly used traditionally for the treatment of diabetes in Morocco.
This current investigation aimed to assess the antihyperglycemic capacity of the aqueous extract of Collard green leaves.
The effect of a single (6 hours) and repeated (seven days of treatment) oral administration of Collard green aqueous extract (CGAE) at a dose of 60 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of liver was carried out according to the Hematoxylin-Eosin method. Furthermore, a preliminary phytochemical screening, and the quantification of phenolic, flavonoid and tannins contents as well as the antioxidant activity using DPPH assay were carried out.
The results demonstrated that Collard green A.E. (CGAE) exhibited a significant antihyperglycemic effect and positive improvement liver histology in diabetic rats. After a single oral administration of CGAE, blood glucose levels were lowered from 15±2 mM to 4.9±1 mM (p<0.0001) at the sixth hour in diabetic rats but no change was observed in normal rats. Repeated CGAE administration was able to reduce blood glucose levels from 15±2 mM to normal values (5±1 mM at the seventh day, p<0.0001) while the extract had no effect in normal rats. In addition, we revealed that CGAE is rich in several phytochemical compounds and exerts an important antioxidant activity.
In conclusion, this study demonstrates that Collard green possesses a beneficial effect against diabetes and can be used in the program diet of diabetic patients.
本研究旨在评估甘蓝的降血糖活性。
羽衣甘蓝或甘蓝变种，属于十字花科，在“强力蔬菜”中排名第十，在摩洛哥传统上常用于治疗糖尿病。
本研究旨在评估羽衣甘蓝叶水提物的降血糖能力。
单次（6 小时）和重复（7 天治疗）口服 60mg/kg 羽衣甘蓝水提物（CGAE）对正常和链脲佐菌素诱导的糖尿病大鼠血糖和血脂谱的影响进行了研究。此外，根据苏木精-伊红法对肝脏进行了组织病理学检查。此外，还进行了初步的植物化学成分筛选，以及使用 DPPH 测定法对酚类、类黄酮和单宁含量和抗氧化活性进行了定量。
结果表明，羽衣甘蓝 A.E.（CGAE）对糖尿病大鼠表现出显著的降血糖作用和改善肝脏组织学的作用。单次口服 CGAE 后，糖尿病大鼠的血糖水平从 15±2mM 降低至 4.9±1mM（p<0.0001），而在正常大鼠中没有观察到变化。重复 CGAE 给药能够将糖尿病大鼠的血糖水平从 15±2mM 降低至正常水平（第 7 天 5±1mM，p<0.0001），而提取物对正常大鼠没有影响。此外，我们发现 CGAE 富含几种植物化学成分，并具有重要的抗氧化活性。
综上所述，本研究表明甘蓝具有有益的抗糖尿病作用，可用于糖尿病患者的饮食计划。
