希腊石蚕水提取物对链脲佐菌素诱导的糖尿病大鼠的降血糖活性
Antihyperglycemic Activity of Micromeria graeca Aqueous Extract in Streptozotocin-Induced Diabetic Rats.
作者信息
Akdad Mourad, Azzane Amine, El Ouady Fadwa, Moujani Abdelhadi, El Khallouki Farid, Eddouks Mohamed
机构信息
Department of Ethnopharmacology and Pharmacognosy, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, Errachidia, 52000, Morocco.
出版信息
Endocr Metab Immune Disord Drug Targets. 2021;21(5):887-894. doi: 10.2174/1871530320666200807124940.
AIM
This study aimed to investigate the effect of Micromeria graeca on blood glucose levels and lipid parameters in an experimental model of diabetes.
BACKGROUND
Micromeria graeca (L.) Benth. ex Rchb is a medicinal plant used in Morocco for the treatment of several pathologies including diabetes.
OBJECTIVE
This study aimed to evaluate the antihyperglycemic and antihyperlipidemic effects of the aqueous extract of Micromeria graeca (M. graeca) aerial parts (AEMG) under physiological (normal rats) and pathological (STZ-induced diabetic rats) conditions. Additionally, we analyzed the phytochemical composition and antioxidant capacity.
METHODS
The effects of the acute and sub-chronic administration of AEMG (20 mg/kg) on blood glucose levels and lipid profiles were evaluated in normal and streptozotocin-induced diabetic rats. Moreover, the phytochemical analysis was carried with standard tests and estimation of total phenolics compounds by Folin-Ciocalteu reagent. The antioxidant activity was realized by the DPPH method.
RESULTS
Single oral administration of M. graeca aqueous extract decreased blood glucose levels 4 and 6 hours (p<0.01) after treatment in diabetic rats. In accordance, the repeated oral administration of M. graeca showed a significant reduction in blood glucose levels in diabetic rats since the second day to the end of the period experiment (p<0.0001). In addition, two weeks of treatment with M. graeca reduced total cholesterol levels (p<0.05) with a significant increase of HDL-c level (p<0.01) in diabetic rats. Moreover, M. graeca scavenged DPPH radical in a dose-dependent manner (IC50=0.48 mg/ml), whereas IC50 was 0.55 mg/ml for BHT. Phytochemical analysis showed the richness of Micromeria graeca on polyphenols (281.94±4.61 mg GAE/1 g), flavonoids, tannins, glycosides, saponins, sterols, sesquiterpenes, and terpenoids.
CONCLUSION
AEMG exhibits antihyperglycemic and antihyperlipidemic activities in STZ-induced diabetic rats and a potent antioxidant capacity.
目的
本研究旨在探讨希腊百里香对糖尿病实验模型中血糖水平和血脂参数的影响。
背景
希腊百里香（Micromeria graeca (L.) Benth. ex Rchb）是一种在摩洛哥用于治疗包括糖尿病在内的多种病症的药用植物。
目的
本研究旨在评估希腊百里香地上部分水提取物（AEMG）在生理（正常大鼠）和病理（链脲佐菌素诱导的糖尿病大鼠）条件下的降血糖和降血脂作用。此外，我们分析了其植物化学成分和抗氧化能力。
方法
评估了AEMG（20毫克/千克）急性和亚慢性给药对正常和链脲佐菌素诱导的糖尿病大鼠血糖水平和血脂谱的影响。此外，采用标准试验进行植物化学分析，并用福林-西奥尔特试剂估算总酚类化合物。通过DPPH法实现抗氧化活性测定。
结果
单次口服希腊百里香水提取物可使糖尿病大鼠治疗后4小时和6小时的血糖水平降低（p<0.01）。相应地，反复口服希腊百里香显示，从实验期第二天到实验结束，糖尿病大鼠的血糖水平显著降低（p<0.0001）。此外，用希腊百里香治疗两周可降低糖尿病大鼠的总胆固醇水平（p<0.05），同时显著提高高密度脂蛋白胆固醇水平（p<0.01）。此外，希腊百里香以剂量依赖方式清除DPPH自由基（IC50=0.48毫克/毫升），而丁基羟基甲苯的IC50为0.55毫克/毫升。植物化学分析表明，希腊百里香富含多酚（281.94±4.61毫克没食子酸当量/1克）、黄酮类、单宁、糖苷、皂苷、甾醇、倍半萜和萜类。
结论
AEMG在链脲佐菌素诱导的糖尿病大鼠中表现出降血糖和降血脂活性以及强大的抗氧化能力。
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