Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, SBFis, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil.
Department of Physiotherapy, São Paulo State University (UNESP), Presidente Prudente, São Paulo, Brazil.
Sci Rep. 2020 May 13;10(1):7937. doi: 10.1038/s41598-020-64683-0.
The objective of this study is to identify whether oxytocin (OT) contributes to the reduction of osteopenia in the femoral neck of rats in periestropause. Animals in irregular estrous cycles received two NaCl injections (0.15 mol/L) or OT (134 μg/kg) over a 12-h interval, and after thirty-five days without treatments, the biological sample collection was performed. The oxytocin group (Ot) demonstrated the highest enzymatic activity of alkaline phosphatase (p = 0.0138), lowest enzymatic activity of tartrate-resistant acid phosphatase (p = 0.0045), higher percentage of compact bone (p = 0.0359), cortical expression of runt-related transcription factor 2 (p = 0.0101), osterix (p = 0.0101), bone morphogenetic protein-2/4 (p = 0.0101) and periostin (p = 0.0455). Furthermore, the mineral-to-matrix ratio (νPO/Proline) was higher and type-B carbonate substitution (CO/νPO) was lower (p = 0.0008 and 0.0303) in Ot group. The Ot showed higher areal bone mineral density (p = 0.0050), cortical bone area (p = 0.0416), polar moment of inertia, maximum, minimum (p = 0.0480, 0.0480, 0.0035), bone volume fraction (p = 0.0166), connectivity density (p < 0.0001), maximal load (p = 0.0003) and bone stiffness (p = 0.0145). In Ot percentage of cortical pores (p = 0.0102) and trabecular number (p = 0.0088) was lower. The results evidence action of OT in the reduction of osteopenia, suggesting that it is a promising anabolic strategy for the prevention of primary osteoporosis during the periestropause period.
这项研究的目的是确定催产素 (OT) 是否有助于减少围绝经期大鼠股骨颈的骨质疏松症。不规则动情周期的动物在 12 小时的间隔内接受两次 NaCl 注射 (0.15 mol/L) 或 OT (134 μg/kg)，在 35 天没有治疗后，进行生物样本采集。催产素组 (Ot) 表现出碱性磷酸酶的最高酶活性 (p=0.0138)，抗酒石酸酸性磷酸酶的最低酶活性 (p=0.0045)，较高的致密骨百分比 (p=0.0359)， runt 相关转录因子 2 的皮质表达 (p=0.0101)，osterix(p=0.0101)，骨形态发生蛋白-2/4(p=0.0101)和骨膜蛋白(p=0.0455)。此外，Ot 组的矿物质/基质比 (νPO/Proline) 更高，B 型碳酸盐取代 (CO/νPO) 更低 (p=0.0008 和 0.0303)。Ot 组的面积骨矿物质密度 (p=0.0050)、皮质骨面积 (p=0.0416)、极惯性矩、最大、最小 (p=0.0480、0.0480、0.0035)、骨体积分数 (p=0.0166)、连通密度 (p<0.0001)、最大负荷 (p=0.0003) 和骨刚度 (p=0.0145) 更高。在 Ot 中，皮质孔的百分比 (p=0.0102) 和小梁数 (p=0.0088) 较低。结果表明 OT 可减少骨质疏松症，表明其可能成为围绝经期原发性骨质疏松症预防的有前途的合成代谢策略。
