Kim Jung-Eun, Lee Nam Hyouck, Kim Young Ho, Kim Young Eon, Lim Tae-Gyu, Song Kyung-Mo
Research Group of Food Processing Korea Food Research Institute Wanju-gun Korea.
SME Solution Center Korea Food Research Institute Wanju-gun Korea.
Food Sci Nutr. 2020 Apr 8;8(5):2205-2213. doi: 10.1002/fsn3.1336. eCollection 2020 May.
The fruit of L. (Arctii Fructus) is one of the most popularly used medicinal plant components in Asia. To enhance the functionality of Arctii Fructus extract, a bioconversion method was developed to produce arctigenin from arctiin. Treatment with β-glucosidase increased the arctigenin content by >5 fold in Arctii Fructus extracts. The bioconversion products enhanced the apoptosis of cancer cells. The cell viabilities of gefitinib-resistant lung cancer HCC827 (HCC827GR) cells and colon cancer cells (DLD1) were decreased by 40% and 35%, respectively. The bioconversion products also decreased anchorage-independent growth of cancer cells. In addition, the increase of apoptosis in cancer cells by bioconversion was confirmed by the flow cytometry analysis. These results indicated that arctigenin exerts anticancer effects on lung and colon cancer cells and that Arctii Fructus can potentially function as a chemopreventive agent. In addition, bioconverted Arctii Fructus extract displayed higher anticancer activity than the same levels of purified arctigenin, indicating the advantage of consuming Arctii Fructus itself as a food or medicinal material.
牛蒡子果实是亚洲最常用的药用植物成分之一。为了增强牛蒡子提取物的功能，开发了一种生物转化方法，从牛蒡子苷生产牛蒡子苷元。用β-葡萄糖苷酶处理使牛蒡子提取物中的牛蒡子苷元含量增加了5倍以上。生物转化产物增强了癌细胞的凋亡。吉非替尼耐药的肺癌HCC827（HCC827GR）细胞和结肠癌细胞（DLD1）的细胞活力分别降低了40%和35%。生物转化产物还降低了癌细胞的非锚定依赖性生长。此外，通过流式细胞术分析证实了生物转化导致癌细胞凋亡增加。这些结果表明，牛蒡子苷元对肺癌和结肠癌细胞具有抗癌作用，牛蒡子有可能作为一种化学预防剂发挥作用。此外，生物转化的牛蒡子提取物显示出比相同水平的纯化牛蒡子苷元更高的抗癌活性，这表明将牛蒡子本身作为食物或药物材料食用具有优势。
