Mahmoudvand Hossein, Tavakoli Kareshk Amir, Nabi Moradi Mohammad, Monzote Fidalgo Lianet, Mirbadie Seyed Reza, Niazi Massumeh, Khatami Mehrdad
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Laboratory Sciences, School of Allied Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Iran J Parasitol. 2020 Jan-Mar;15(1):22-30.
Broad spectrums of pharmacological properties, including antimicrobial activity have been attributed to Boiss (Laminaceae). The in vivo efficacy and safety of essential oil (ZM-EO) were evaluated against acute toxoplasmosis caused by (Sarcocystidae) in mice.
(aerial parts) was obtained from the rural districts of Kerman city (Kerman Province) Southwestern Iran, in May of 2016. Male NMRI mice were orally treated with normal saline (control group) and ZM-EO at the doses of 0.2 and 0.4 mL/kg once a day for 14 d (8 mice in each group) On the 15 day, the mice were infected with 104 tachyzoites of RH strain by intraperitoneal route. The mortality rate and parasite load were determined in the infected mice. Additionally, 24 mice were applied to examine the sub-acute toxicity of ZM-EO at the above doses after treatment during 14 d.
GC/MS analysis displayed that the key constituents were thymol (45.4%), carvacrol (23%) and -cymene (10.6%), respectively. Overall, 100% mortality was observed on the 8 and 9 days in treated mice with the concentrations of 0.2 and 0.4 mL/kg, respectively. The mean number of tachyzoites in the mice treated with 0.2 and 0.4 mL/kg of ZM-EO were 189×10 and 76×10 cell/mL, respectively, meaningfully (<0.05) reduced compared with the control mice. Results also demonstrated that ZM-EO had no important toxicity on mice.
The results demonstrated the efficacy of ZM-EO against acute toxoplasmosis. Nevertheless, supplementary surveys are mandatory to examine its precise effects, mainly immunomodulatory effect on toxoplasmosis.
薄荷属（唇形科）具有广泛的药理特性，包括抗菌活性。本研究评估了薄荷属植物挥发油（ZM - EO）对小鼠急性弓形虫病（由弓形虫属（肉孢子科）引起）的体内疗效和安全性。
2016年5月从伊朗西南部克尔曼市（克尔曼省）农村地区采集薄荷属植物地上部分。雄性NMRI小鼠口服生理盐水（对照组）和ZM - EO，剂量分别为0.2和0.4 mL/kg，每天一次，共14天（每组8只小鼠）。在第15天，通过腹腔途径给小鼠感染104个RH株速殖子。测定感染小鼠的死亡率和寄生虫载量。此外，24只小鼠用于检测上述剂量的ZM - EO在治疗14天后的亚急性毒性。
气相色谱/质谱分析显示，主要成分分别为百里香酚（45.4%）、香芹酚（23%）和对伞花烃（10.6%）。总体而言，分别用浓度为0.2和0.4 mL/kg的ZM - EO处理的小鼠在第8天和第9天观察到100%的死亡率。用0.2和0.4 mL/kg ZM - EO处理的小鼠体内速殖子的平均数量分别为189×10和76×10个细胞/mL，与对照小鼠相比有显著降低（<0.05）。结果还表明ZM - EO对小鼠无明显毒性。
结果证明了ZM - EO对急性弓形虫病的疗效。然而，必须进行补充研究以检查其确切作用，主要是对弓形虫病的免疫调节作用。
