Amin Ghobadi Mohammad, Mahmoudvand Hossein, Khalaf Amal Khudair, Azadbakht Fatemeh, Rashidipour Marzieh, Salimikia Iraj
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Iran J Parasitol. 2025 Jan-Mar;20(1):100-110. doi: 10.18502/ijpa.v20i1.18110.
Given the significant role of chitosan nanoparticles in medicine, the present study aimed to assess the efficacy of synthesized chitosan nanoparticles coated with thymol (CNCT) in combating infection.
Mice were administered CNCT orally at dosages ranging from 0.25 to 0.75 mg/kg/day for a duration of 14 days. Following this treatment, they were infected with tachyzoites of the Rh strain to induce acute toxoplasmosis. Then, the mortality rate, parasite load, antioxidant activity, and the gene expression level of proinflammatory cytokines were evaluated.
The dimensions of CNTN exhibit variability, with a mean size of 295 nm. The prophylactic administration of CNTN in mice infected with T. gondii resulted in a significant enhancement in survival rates and a considerable decrease in parasite load (<0.001). The CNTN caused a significant decrease in malondialdehyde level, while a notable increase ( < 0.001) in the activity of the antioxidant enzymes. The feeding the mice infected with CNTN caused a meaningful elevation in the expression level of TNFα- and IL-1β (<0.001). The biochemical analyses indicated no significant changes in the serum levels of liver and kidney function markers.
The recent study revealed that CNTN demonstrates promising effects against toxoplasmosis in murine models. These effects are attributed to its antioxidant properties and immunomodulatory capabilities, which increase specific pro-inflammatory cytokines without any noticeable signs of toxicity to liver and kidney function.
鉴于壳聚糖纳米颗粒在医学中的重要作用，本研究旨在评估合成的百里香酚包被壳聚糖纳米颗粒（CNCT）对抗感染的效果。
小鼠口服CNCT，剂量范围为0.25至0.75毫克/千克/天，持续14天。此治疗后，它们感染Rh株速殖子以诱导急性弓形虫病。然后，评估死亡率、寄生虫载量、抗氧化活性和促炎细胞因子的基因表达水平。
CNTN的尺寸具有变异性，平均大小为295纳米。对感染弓形虫的小鼠预防性给予CNTN导致存活率显著提高，寄生虫载量显著降低（<0.001）。CNTN使丙二醛水平显著降低，而抗氧化酶活性显著增加（<0.001）。给感染CNTN的小鼠喂食导致TNFα和IL-1β的表达水平有意义地升高（<0.001）。生化分析表明肝功能和肾功能标志物的血清水平无显著变化。
最近的研究表明，CNTN在小鼠模型中对弓形虫病显示出有前景的效果。这些效果归因于其抗氧化特性和免疫调节能力，其增加特定促炎细胞因子而对肝功能和肾功能没有任何明显的毒性迹象。
