Kulu Irem, Huang Rui, Kalyanaraman Bhavna, Rotello Vincent M
Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, MA, 01003, United States.
Department of Chemistry, Faculty of Science, Gebze Technical University,41400 Gebze, Kocaeli, Turkey.
MethodsX. 2020 Apr 21;7:100896. doi: 10.1016/j.mex.2020.100896. eCollection 2020.
2 nm gold nanoparticles (AuNPs) have promising applications within drug and protein delivery, bioimaging, and biosensing. By performing ligand place-exchange reactions, AuNPs protected with alkanethiolate ligands can be functionalized to regulate their behaviors. In this reaction, a new ligand is incorporated by mixing a thiol with the AuNPs. To remove the excess new ligand as well as the displaced thiolate, dialysis has previously been the most widely used method. However, this purification method is time-consuming and fails to remove unwanted thiols completely. In this study, we describe a fast and efficient procedure to purify AuNP aqueous solution through liquid-liquid extraction using dichloromethane.•We demonstrate a facile way to purify AuNPs after ligand place-exchange reactions through liquid-liquid extraction.•Liquid-liquid extraction is a simple, inexpensive and efficient method for AuNP purification.•This protocol enables us to completely purify AuNPs in a few hours and can be used as a much quicker and more scaleable valid alternative to dialysis.
2纳米金纳米颗粒（AuNPs）在药物和蛋白质递送、生物成像及生物传感领域有着广阔的应用前景。通过进行配体置换反应，用链烷硫醇酸盐配体保护的AuNPs可以被功能化以调控其行为。在该反应中，通过将硫醇与AuNPs混合引入新的配体。为了去除过量的新配体以及被取代的硫醇盐，透析此前一直是使用最广泛的方法。然而，这种纯化方法耗时且无法完全去除不需要的硫醇。在本研究中，我们描述了一种通过使用二氯甲烷进行液 - 液萃取来快速高效地纯化AuNP水溶液的方法。
• 我们展示了一种通过液 - 液萃取在配体置换反应后纯化AuNPs的简便方法。
• 液 - 液萃取是一种用于AuNP纯化的简单、廉价且高效的方法。
• 该方案使我们能够在数小时内完全纯化AuNPs，并且可以作为比透析更快且更具扩展性的有效替代方法。
