Department of Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Korea.
Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Korea.
Genes (Basel). 2020 May 13;11(5):545. doi: 10.3390/genes11050545.
Advanced liver fibrosis is the most important prognostic factor in nonalcoholic fatty liver disease (NAFLD). The long noncoding RNA (lncRNA), growth arrest-specific transcript 5 (GAS5), is associated with the inhibition of liver fibrogenesis, and its levels are decreased in cirrhotic liver.
We analyzed 51 patients with NAFLD, the diagnosis of which was confirmed by liver biopsy. Expression of GAS5 in both the liver and plasma of the patients was analyzed using a quantitative real-time polymerase chain reaction according to the fibrosis stage.
Plasma GAS5 expression was significantly higher in patients with advanced fibrosis than in those without. As the fibrosis progressed, GAS5 expression in plasma increased, with the exception of that in cirrhotic livers. Plasma levels of GAS5 were lower in patients with cirrhosis than in those with advanced fibrosis.
Elevated circulating levels of the lncRNA GAS5 are associated with the progression of liver fibrosis prior to the development of cirrhosis.
非酒精性脂肪性肝病（NAFLD）中，肝纤维化进展是最重要的预后因素。长链非编码 RNA（lncRNA）生长停滞特异性转录物 5（GAS5）与肝纤维化抑制有关，在肝硬化肝脏中其水平降低。
我们分析了 51 例经肝活检证实的 NAFLD 患者，根据纤维化分期，采用实时定量聚合酶链反应分析患者肝和血浆中 GAS5 的表达。
进展性肝纤维化患者的血浆 GAS5 表达明显高于无进展性肝纤维化患者。随着纤维化的进展，血浆中 GAS5 的表达增加，但肝硬化肝脏除外。肝硬化患者的血浆 GAS5 水平低于进展性肝纤维化患者。
lncRNA GAS5 的循环水平升高与肝硬化发生前肝纤维化的进展有关。
