Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Sci Rep. 2020 May 15;10(1):8035. doi: 10.1038/s41598-020-64559-3.
Salmonella infections have become a major health concern in recent decades. This pathogen has evolved to become resistant to antibiotics, which has caused problems in its treatment. As such, finding a novel preventive method is important in the treatment and management of this infection. In recent years, uses of probiotics, especially spore-former genera such as Bacillus spp. has become increasingly popular. In this study spores of two probiotic bacteria, Bacillus subtilis and Bacillus coagulans were fed to rats for three weeks through their daily water intake after which Salmonella Typhimurium was gavaged to the rats. On days 1, 3, 5 and 7 after gavaging, the number of Salmonella was counted in liver, spleen, mesenteric lymph nodes, feces and content of ileum and cecum. Hematological and biochemical parameters, inflammatory mediators, total antioxidant capacity and malondialdehyde were also measured. The results showed that B. subtilis and B. coagulans caused delation in infiltration of Salmonella into the lymph nodes, spleen and liver, reduction of the inflammatory mediators, and decreases in oxidative stress, hematological and biochemical changes. The overall count of Salmonella in the above mentioned parameters has also decreased and a faster return to normal base were also witnessed. The results showed that the use of B. subtilis and B. coagulans can potentially help boost the body's immune system, to combat the effects of exposure to the Salmonella pathogen.
在最近几十年，沙门氏菌感染已经成为一个主要的健康问题。这种病原体已经进化到对抗生素产生耐药性，这在其治疗中造成了问题。因此，寻找一种新的预防方法对于这种感染的治疗和管理非常重要。近年来，益生菌的使用，特别是芽孢形成菌属如芽孢杆菌属的使用变得越来越流行。在这项研究中，两种益生菌细菌的孢子，枯草芽孢杆菌和凝结芽孢杆菌，通过它们的日常饮水被喂给大鼠，持续 3 周，然后用鼠伤寒沙门氏菌给大鼠灌胃。在灌胃后的第 1、3、5 和 7 天，计算了肝、脾、肠系膜淋巴结、粪便和回肠和盲肠内容物中沙门氏菌的数量。还测量了血液学和生化参数、炎症介质、总抗氧化能力和丙二醛。结果表明，枯草芽孢杆菌和凝结芽孢杆菌导致沙门氏菌向淋巴结、脾脏和肝脏的浸润扩张，炎症介质减少，氧化应激、血液学和生化变化减少。上述参数中沙门氏菌的总计数也减少了，并且更快地恢复到正常基础。结果表明，使用枯草芽孢杆菌和凝结芽孢杆菌可以潜在地帮助增强机体的免疫系统，对抗暴露于沙门氏菌病原体的影响。
