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罗勒的酚类含量、抗氧化能力以及α-淀粉酶和α-葡萄糖苷酶抑制活性

Phenolic content, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities of Lour.

作者信息

Wang Zhuo, Gao Xiaoxv, Li Wenfeng, Tan Si, Zheng Qiaoran

机构信息

1Forestry College, Beihua University, Jilin, 132013 China.

2School of Advanced Agriculture and Bioengineering, Yangtze Normal University, No. 16 Juxian Avenue, Fuling District, Chongqing, 408100 China.

出版信息

Food Sci Biotechnol. 2019 Dec 23;29(5):683-692. doi: 10.1007/s10068-019-00708-3. eCollection 2020 May.

Abstract

The aim of this study was to compare the phenolic compound profiles and antioxidant capacities of eight varieties of longan ( Lour.) planted in the middle and upper Yangtze River area. The total polyphenols content (TPC) and total flavonoids content (TFC) of dried longan pulp ranged from 1.07 ± 0.05 to 1.22 ± 0.05 mg gallic acid equivalents/g and 0.25 ± 0.07 to 0.87 ± 0.14 mg rutin equivalents/g. UHPLC-QqQ-MS/MS analysis revealed 12 individual polyphenol compounds in longan. The Fuwan8, Dongliang and FD97 varieties showed the strongest DPPH scavenging activity (IC of 1.03 g/mL). The highest ABTS scavenging activity was found in the Dongliang. In the enzyme assays, the Fuwan8 and Dongliang varieties demonstrated maximum α-amylase and α-glucosidase inhibition activities, with values of 97.56 and 88.58%, respectively. The principal component analysis indicated that the Rongyu8 and Songfengben cultivars have nearly analogous polyphenol compounds.

摘要

本研究旨在比较长江中上游地区种植的8个龙眼品种的酚类化合物谱和抗氧化能力。干龙眼果肉的总多酚含量(TPC)和总黄酮含量(TFC)范围分别为1.07±0.05至1.22±0.05毫克没食子酸当量/克和0.25±0.07至0.87±0.14毫克芦丁当量/克。超高效液相色谱-四极杆-线性离子阱串联质谱(UHPLC-QqQ-MS/MS)分析表明,龙眼中共鉴定出12种酚类化合物。福丸8号、东良和FD97品种表现出最强的1,1-二苯基-2-三硝基苯肼(DPPH)清除活性(半数抑制浓度(IC)为1.03克/毫升)。东良的2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)清除活性最高。在酶活性测定中,福丸8号和东良品种表现出最大的α-淀粉酶和α-葡萄糖苷酶抑制活性,分别为97.56%和88.58%。主成分分析表明,荣玉8号和松风本品种的多酚化合物几乎相似。

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