Balasundaram Ghayathri, Goh Yonggeng, Moothanchery Mohesh, Attia Amalina, Lim Hann Qian, Burton Neal C, Qiu Yi, Putti Thomas Choudary, Chan Ching Wan, Hartmann Mikael, Quek Swee Tian, Olivo Malini
Laboratory of Bio-Optical Imaging, Singapore Bioimaging Consortium, Singapore.
Department of Diagnostic Imaging, National University Hospital, Singapore.
Photoacoustics. 2020 Apr 27;19:100164. doi: 10.1016/j.pacs.2020.100164. eCollection 2020 Sep.
In this pilot study, we tested an ultrasound-guided optoacoustic tomography (US-OT) two-dimensional (2D) array scanner to understand the optoacoustic patterns of excised breastconserving surgery (BCS) specimens. We imaged 14 BCS specimens containing malignant tumors at eight wavelengths spanning 700-1100 nm. Spectral unmixing across multiple wavelengths allowed for visualizing major intrinsic chromophores in the breast tissue including hemoglobin and lipid up to a depth of 7 mm. We identified less/no lipid signals within the tumor and intense deoxy-hemoglobin (Hb) signals on the rim of the tumor as unique characteristics of malignant tumors in comparison to no tumor region. We also observed continuous broad lipid signals as features of negative margins and compromised lipid signals interrupted by vasculature as features of positive margins. These differentiating patterns can form the basis of US-OT to be explored as an alternate, fast and efficient intraoperative method for evaluation of tumor resection margins.
在这项初步研究中，我们测试了一种超声引导光声断层扫描（US-OT）二维（2D）阵列扫描仪，以了解保乳手术（BCS）切除标本的光声模式。我们对14个含有恶性肿瘤的BCS标本进行了成像，成像波长范围为700-1100nm，共8个波长。跨多个波长的光谱解混使得能够可视化乳腺组织中主要的内源性发色团，包括血红蛋白和脂质，深度可达7mm。与无肿瘤区域相比，我们发现肿瘤内脂质信号较少/无脂质信号，肿瘤边缘有强烈的脱氧血红蛋白（Hb）信号是恶性肿瘤的独特特征。我们还观察到连续的宽脂质信号是阴性切缘的特征，而被血管中断的受损脂质信号是阳性切缘的特征。这些区分模式可以成为US-OT的基础，作为一种替代的、快速有效的术中评估肿瘤切除边缘的方法进行探索。
