Cárcamo-Martínez Álvaro, Anjani Qonita Kurnia, Permana Andi Dian, Cordeiro Ana Sara, Larrañeta Eneko, Donnelly Ryan F
School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK.
Department of Pharmaceutics, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia.
Int J Pharm X. 2020 May 4;2:100048. doi: 10.1016/j.ijpx.2020.100048. eCollection 2020 Dec.
Two groups of single polymeric needles (crosslinked Gantrez®S-97 and poly(ethylene glycol)) of different lengths (2 mm and 4.5 mm) with defined base widths were fabricated and tested in terms of their mechanical strength and insertion abilities using two skin models (Parafilm® and porcine skin). For the shorter needles, application of an axial force (32 N) resulted in a height reduction of approximately 80%. Nonetheless, around 80% of total needle length was successfully inserted in both skin models. Optical coherence tomography showed that base width highly impacted insertion capabilities of the longer needles as only the thicker one (0.922 mm width at base) inserted into porcine skin. Additionally, needles were coated with rhodamine B and inserted into porcine skin. In comparison to a control, penetration depth of the model drug increased 2-fold for short and 4.5-fold for long needles, respectively. Moreover, quantification across skin sections showed that shorter needles delivered 10 μg of the compound in a depth of 1.5-2.0 mm while long needles were capable of delivering 5 μg into even deeper skin layers (2.0-3.0 mm), confirming the potential of coated polymeric needles for rapid and deep intradermal delivery.
制备了两组不同长度（2毫米和4.5毫米）、具有确定基部宽度的单一聚合物针（交联的Gantrez®S - 97和聚乙二醇），并使用两种皮肤模型（Parafilm®和猪皮）对其机械强度和插入能力进行了测试。对于较短的针，施加轴向力（32牛）会导致高度降低约80%。尽管如此，在两种皮肤模型中，约80%的针总长度成功插入。光学相干断层扫描显示，基部宽度对较长针的插入能力有很大影响，因为只有较厚的针（基部宽度为0.922毫米）能插入猪皮。此外，将针用罗丹明B包被后插入猪皮。与对照相比，模型药物的穿透深度对于短针增加了2倍，对于长针增加了4.5倍。而且，对皮肤切片的定量分析表明，较短的针在1.5 - 2.0毫米深度递送10微克化合物，而长针能够将5微克递送至更深的皮肤层（2.0 - 3.0毫米），证实了包被聚合物针用于快速和深层皮内递送的潜力。
