University of Chinese Academy of Sciences, 100049, Beijing, PR China.
Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
Int J Syst Evol Microbiol. 2020 Jun;70(6):3633-3638. doi: 10.1099/ijsem.0.004205.
The Gram-stain-negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP390, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP390 was related to the genus and had highest 16S rRNA gene sequence identity with the type strain of EC11 (97.8 %). Sequence similarities to all other type strains of current species of the genus were below 97 %. The predominant cellular fatty acids were iso-C and iso-CG. The quinone system for strain TP390 comprised predominantly menaquinone MK-6 and the polar lipid profile contained phosphatidylethanolamine, four unknown aminolipids, one glycolipid and six unknown polar lipids. The genomic DNA G+C content of strain TP390 was 31.2 mol%. In addition, the maximum values of DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) between strain TP390 with KCTC 42149 were 22.60 and 80.01% respectively. Combined data from phenotypic, phylogenetic, DDH and ANI data demonstrated that the strain TP390 is representative of a novel species of the genus , for which we propose the name sp. nov. (type strain TP390=KACC 18559=CGMCC 1.15398).
革兰氏阴性、杆状、兼性厌氧、能动的细菌菌株，被命名为 TP390，是从热带西太平洋 Yap 海沟附近的一座海山上分离得到的。基于 16S rRNA 基因序列的系统发育分析表明，菌株 TP390 与属有关，与模式菌株 EC11 的 16S rRNA 基因序列相似度最高（97.8 %）。与属内所有其他现有种的模式菌株的序列相似性均低于 97 %。主要的细胞脂肪酸为 iso-C 和 iso-CG。菌株 TP390 的醌类系统主要由menaquinone MK-6 组成，极性脂谱含有磷脂酰乙醇胺、四种未知的胺脂、一种糖脂和六种未知的极性脂。菌株 TP390 的基因组 DNA G+C 含量为 31.2 mol%。此外，菌株 TP390 与 KCTC 42149 的 DNA-DNA 杂交（DDH）和平均核苷酸同一性（ANI）的最大值分别为 22.60%和 80.01%。表型、系统发育、DDH 和 ANI 数据的综合结果表明，该菌株 TP390 代表了属的一个新种，我们建议将其命名为 sp. nov.（模式菌株 TP390=KACC 18559=CGMCC 1.15398）。
