Wang Qian, Liu Feng, Zhang De-Chao
University of Chinese Academy of Sciences, 100049, Beijing, PR China.
Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4569-4575. doi: 10.1099/ijsem.0.004315.
The Gram-stain-negative, orange-pigmented, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated TP-CH-4, was isolated from a seamount near the Yap Trench in the tropical western Pacific. The optimal growth conditions were determined to be at pH 7-8, 25-30 °C and in the presence of 2 % (w/v) NaCl. The major respiratory quinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and three unidentified polar lipids. The predominant cellular fatty acids were iso-C and summed feature 1 (composed of C3-OH and/or iso-CH). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TP-CH-4 was a member of the family and formed a distinct lineage. Strain TP-CH-4 displayed highest sequence similarities to KMM 3531 (95.1 %) and A11 (93.9 %). Genome sequencing revealed the strain TP-CH-4 has a genome size of 4.5 Mbp and a G+C content of 44.5 mol%. Collectively, based on phenotypic, chemotaxonomic, phylogenetic and genomic evidence, strain TP-CH-4 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is TP-CH-4 (=CGMCC 1.17120=KCTC 72434).
革兰氏染色阴性、产橙色色素、不形成芽孢、无运动性、严格需氧的杆状细菌菌株TP-CH-4，是从热带西太平洋雅浦海沟附近的一座海山中分离得到的。确定其最佳生长条件为pH 7-8、25-30℃以及存在2%（w/v）NaCl的环境。主要呼吸醌为MK-6。极性脂质谱包含磷脂酰乙醇胺、两种未鉴定的氨基脂质、两种未鉴定的磷脂和三种未鉴定的极性脂质。主要的细胞脂肪酸为异-C和总和特征1（由C3-OH和/或异-CH组成）。16S rRNA基因序列的系统发育分析表明，菌株TP-CH-4是该科的一个成员，并形成了一个独特的谱系。菌株TP-CH-4与KMM 3531（95.1%）和A11（93.9%）显示出最高的序列相似性。基因组测序表明，菌株TP-CH-4的基因组大小为4.5 Mbp，G+C含量为44.5 mol%。总体而言，基于表型、化学分类、系统发育和基因组证据，菌株TP-CH-4代表了该科一个新属的一个新物种，为此提出新属名 ，新种名 。 的模式菌株为TP-CH-4（=CGMCC 1.17120=KCTC 72434）。
