School of Environment and Civil Engineering, Dongguan University of Technology, Dongguan, Guangdong, 523808, People's Republic of China.
School of Environment and Civil Engineering, Dongguan University of Technology, Dongguan, Guangdong, 523808, People's Republic of China.
Talanta. 2022 Feb 1;238(Pt 1):122991. doi: 10.1016/j.talanta.2021.122991. Epub 2021 Oct 22.
This work presents a novel 3D carbonized wood-based integrated electrochemical immunosensor for ultrasensitive detection of procalcitonin (PCT) antigen at picogram level, achieving a wide linear detection range for PCT concentrations range from 0.05 to 90 pg mL with a low detection limit of 0.014 pg mL (S/N = 3), outperforming the previous reports. 3D carbonized wood as a new immunosensor matrix is used for electrochemical PCT biosensing, improving the stability of electrode and overcoming the disadvantages of traditional glassy carbon electrode (GCE). It obtained an excellent detection result, due to it has abundant mutual crisscross microchannels that promote the reactants and electrons transfer, greatly amplify the current signal. This novel sandwich-type electrochemical immunosensor is composed of 3D carbonized wood, carboxylic multi-walled carbon nanotube (cMWCNT), Au@CoO core-shell nanosphere and Au/single layer nitrogen-doped graphene (Au/SL-NG), when it is applied for PCT detection in real clinical samples, it exhibits high accuracy same as enzyme-linked immunosorbent assay (ELISA) method.
本工作提出了一种新颖的 3D 碳化木材基集成电化学免疫传感器，用于在皮克级水平上超灵敏检测降钙素原 (PCT) 抗原，实现了从 0.05 到 90 pg mL 的宽线性检测范围，检测限低至 0.014 pg mL (S/N = 3)，优于之前的报道。3D 碳化木材作为一种新的免疫传感器基质，用于电化学 PCT 生物传感，提高了电极的稳定性，并克服了传统玻碳电极 (GCE) 的缺点。它获得了出色的检测结果，因为它具有丰富的相互交错的微通道，促进了反应物和电子的转移，大大放大了电流信号。这种新型三明治型电化学免疫传感器由 3D 碳化木材、羧酸多壁碳纳米管 (cMWCNT)、Au@CoO 核壳纳米球和 Au/单层氮掺杂石墨烯 (Au/SL-NG) 组成，当用于实际临床样本中的 PCT 检测时，它表现出与酶联免疫吸附测定 (ELISA) 方法相同的高精度。
