通过工程化解脂耶氏酵母生产植物天然产物。
Production of plant natural products through engineered Yarrowia lipolytica.
机构信息
Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
出版信息
Biotechnol Adv. 2020 Nov 1;43:107555. doi: 10.1016/j.biotechadv.2020.107555. Epub 2020 May 15.
Plant natural products have broad applications in the pharmaceutical, food, cosmetics and flavor industries. However, the current manufacturing methods for plant natural products mainly rely on plant extraction and chemical synthesis, which are associated with unsustainability and severe environmental problems. The advancement of genetic technology has facilitated the engineering of microbes for producing plant natural products, but their industrial production titers remain low. Yarrowia lipolytica, a nonconventional yeast, is a promising and superior host for plant natural products manufacture, due to its advanced properties compared to conventional hosts. In this review, metabolic engineering approaches recently employed for overproduction of useful plant natural products, including terpenoids and polyketides, in Y. lipolytica are summarized. The current challenges for the biosynthesis of typical compounds are discussed and promising engineering strategies for future work are also suggested. This review provides guidance for engineering Y. lipolytica as a robust host for plant natural products production.
植物天然产物在制药、食品、化妆品和香料行业有广泛的应用。然而，目前植物天然产物的制造方法主要依赖于植物提取和化学合成，这与不可持续性和严重的环境问题有关。遗传技术的进步促进了微生物生产植物天然产物的工程改造，但它们的工业生产产量仍然很低。与传统宿主相比，脂肪假丝酵母（Yarrowia lipolytica）作为一种非传统酵母，是制造植物天然产物的有前途和优越的宿主。在这篇综述中，总结了最近在脂肪假丝酵母中用于生产有用植物天然产物（包括萜类化合物和聚酮化合物）的过量生产的代谢工程方法。讨论了典型化合物生物合成的当前挑战，并为未来的工作提出了有前途的工程策略。本综述为将脂肪假丝酵母工程化为生产植物天然产物的强大宿主提供了指导。
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