Zhang Jinhao, Wei Lanfang, Yang Jun, Ahmed Waqar, Wang Yating, Fu Lina, Ji Guanghai
Key Laboratory of Agriculture Biodiversity for Plant Disease Management Under the Ministry of Education, Yunnan Agricultural University, Kunming, China.
Agriculture and Rural Affairs Committee of Fengdu County, Chongqing, China.
Front Microbiol. 2020 Apr 30;11:701. doi: 10.3389/fmicb.2020.00701. eCollection 2020.
Root-rot disease caused by is a growing problem in agriculture for commercial cultivation of Diverse microbes colonize plant roots, and numerous earlier studies have characterized the rhizospheric microbiome of ; nevertheless, the function of probiotic consortia on the rhizospheric microbiome against the root-rot disease remain elusive. We have compared and described the rhizospheric microbiome of lightly and severely diseased as well as the interactions of the probiotic consortia and rhizospheric microbiome, and their function to alleviate the plant diseases were explored by inoculating probiotic consortia in bulk soil. From the perspective of microbial diversity, the rhizospheric dominant bacterial and fungal genera were utterly different between lightly and severely diseased plants. Through inoculating assembled probiotic consortia to diseased plant roots, we found that the application of probiotic consortia reshaped the rhizosphere microbiome, increasing the relative abundance of bacteria and fungi, while the relative abundance of potential pathogens was decreased significantly. We developed a microcosm system that provides a preliminary ecological framework for constructing an active probiotic community to reshape soil microbiota and restrain the disease. Microbial community structure differs between lightly and seriously diseased plants. The application of probiotic consortia changes the imbalance of micro-ecology to a state of relative health, reducing plant mortality. Plant disease suppression may be achieved by seeking and applying antagonistic microbes based on their direct inhibitory capability or by restructuring the soil microbiome structure and function.
由[病原体名称未给出]引起的根腐病在[植物名称未给出]的商业种植中，已成为农业领域日益严重的问题。多种微生物定殖于植物根系，众多早期研究已对[植物名称未给出]的根际微生物组进行了表征；然而，益生菌组合对根际微生物组抵御根腐病的功能仍不清楚。我们比较并描述了病情较轻和较重的[植物名称未给出]的根际微生物组，以及益生菌组合与根际微生物组的相互作用，并通过在大田土壤中接种益生菌组合来探索它们减轻植物病害的功能。从微生物多样性的角度来看，病情较轻和较重的植物根际优势细菌和真菌属完全不同。通过将组装好的益生菌组合接种到患病植物根系，我们发现益生菌组合的应用重塑了根际微生物组，增加了细菌和真菌的相对丰度，而潜在病原体的相对丰度则显著降低。我们开发了一个微观系统，为构建一个活跃的益生菌群落以重塑土壤微生物群并抑制病害提供了一个初步的生态框架。病情较轻和较重的植物之间微生物群落结构不同。益生菌组合的应用将微生态的失衡转变为相对健康的状态，降低了植物死亡率。可以通过寻找并应用具有直接抑制能力的拮抗微生物，或通过重组土壤微生物组的结构和功能来实现植物病害抑制。
