Ngnameko Corinne Raïssa, Marchetti Lucia, Zambelli Barbara, Quotadamo Antonio, Roncarati Davide, Bertelli Davide, Njayou Frederic Nico, Smith Stella I, Moundipa Paul F, Costi Maria Paola, Pellati Federica
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy.
Department of Biochemistry, Faculty of Science, The University of Yaounde I, P. Box 812 Yaounde, Cameroon.
Antibiotics (Basel). 2020 May 15;9(5):258. doi: 10.3390/antibiotics9050258.
The medicinal plant P. Beauv. (Bignoniaceae) has been traditionally applied for the prevention and treatment of diseases of the kidney and urinary system, the skin, the gastrointestinal tract, and inflammation in general. The present work shows for the first time how chemical components from this plant inhibit growth by urease inhibition and modulation of virulence factors. The crude extract and the main fractions of bark were tested on isolated strains and the active ones were further fractionated. Fractions and sub-fractions of the plant crude extract were characterized by ultra-high-performance liquid chromatographic tandem high resolution-mass spectrometry detection (UHPLC-HRMS). Several phenolics and triterpenoids were identified. Among the sub-fractions obtained, SB2 showed the capacity to inhibit urease in a heterologous bacterial model. One additional sub-fraction (SE3) was able to simultaneously modulate the expression of two adhesins (HopZ and BabA) and one cytotoxin (CagA). The flavonol kaempferol was identified as the most interesting compound that deserves further investigation as a new hit for its capacity to modulate virulence factors.
药用植物P. Beauv.（紫葳科）传统上一直用于预防和治疗肾脏及泌尿系统疾病、皮肤疾病、胃肠道疾病以及一般性炎症。本研究首次展示了该植物的化学成分如何通过抑制脲酶和调节毒力因子来抑制生长。对树皮的粗提物和主要馏分在分离菌株上进行了测试，并对活性馏分进一步分离。通过超高效液相色谱串联高分辨率质谱检测（UHPLC-HRMS）对植物粗提物的馏分和亚馏分进行了表征。鉴定出了几种酚类和三萜类化合物。在所获得的亚馏分中，SB2在异源细菌模型中显示出抑制脲酶的能力。另一个亚馏分（SE3）能够同时调节两种黏附素（HopZ和BabA）和一种细胞毒素（CagA）的表达。黄酮醇山奈酚被鉴定为最具研究价值的化合物，因其调节毒力因子的能力，作为新的研究热点值得进一步研究。
