Schon Jason, Chahla Jorge, Paudel Sharada, Manandhar Lumanti, Feltham Tyler, Huard Johnny, Philippon Marc, Zhang Zijun
Steadman Philippon Research Institute, Vail, Colorado, USA; Albany Medical College, Albany, New York, New York, USA.
Steadman Philippon Research Institute, Vail, Colorado, USA; Assistant Professor, Rush University Medical Center, Chicago, Illinois, USA.
Bone Joint Res. 2020 May 16;9(4):173-181. doi: 10.1302/2046-3758.94.BJR-2019-0083.R1. eCollection 2020 Apr.
Femoroacetabular impingement (FAI) is a potential cause of hip osteoarthritis (OA). The purpose of this study was to investigate the expression profile of matrix metalloproteinases (MMPs) in the labral tissue with FAI pathology.
In this study, labral tissues were collected from four FAI patients arthroscopically and from three normal hips of deceased donors. Proteins extracted from the FAI and normal labrums were separately applied for MMP array to screen the expression of seven MMPs and three tissue inhibitors of metalloproteinases (TIMPs). The expression of individual MMPs and TIMPs was quantified by densitometry and compared between the FAI and normal labral groups. The expression of selected MMPs and TIMPs was validated and localized in the labrum with immunohistochemistry.
On MMP arrays, most of the targeted MMPs and TIMPs were detected in the FAI and normal labral proteins. After data normalization, in comparison with the normal labral proteins, expression of MMP-1 and MMP-2 in the FAI group was increased and expression of TIMP-1 reduced. The histology of the FAI labrum showed disorderly cell distribution and altered composition of thick and thin collagen fibres. The labral cells expressing MMP-1 and MMP-2 were localized and their percentages were increased in the FAI labrum. Immunohistochemistry confirmed that the percentage of TIMP-1 positive cells was reduced in the FAI labrum.
This study established an expression profile of MMPs and TIMPs in the FAI labrum. The increased expression of MMP-1 and MMP-2 and reduced expression of TIMP-1 in the FAI labrum are indicative of a pathogenic role of FAI in hip OA development. 2020;9(4):173-181.
股骨髋臼撞击症（FAI）是髋关节骨关节炎（OA）的一个潜在病因。本研究的目的是调查伴有FAI病理改变的盂唇组织中基质金属蛋白酶（MMPs）的表达谱。
在本研究中，通过关节镜从4例FAI患者身上采集盂唇组织，并从3例已故供体的正常髋关节获取盂唇组织。分别将从FAI和正常盂唇中提取的蛋白质用于MMP阵列，以筛选7种MMPs和3种金属蛋白酶组织抑制剂（TIMPs）的表达。通过光密度法对单个MMPs和TIMPs的表达进行定量，并在FAI和正常盂唇组之间进行比较。通过免疫组织化学对选定的MMPs和TIMPs的表达进行验证并定位在盂唇中。
在MMP阵列上，大多数靶向的MMPs和TIMPs在FAI和正常盂唇蛋白质中均被检测到。数据归一化后，与正常盂唇蛋白质相比，FAI组中MMP-1和MMP-2的表达增加，而TIMP-1的表达降低。FAI盂唇的组织学显示细胞分布紊乱，粗细胶原纤维组成改变。在FAI盂唇中，表达MMP-1和MMP-2的盂唇细胞被定位且其百分比增加。免疫组织化学证实FAI盂唇中TIMP-1阳性细胞的百分比降低。
本研究建立了FAI盂唇中MMPs和TIMPs的表达谱。FAI盂唇中MMP-1和MMP-2表达增加以及TIMP-1表达降低表明FAI在髋关节OA发展中起致病作用。2020；9(4)：173 - 181。
