Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226021, Jiangsu, P.R. China.
Biosci Rep. 2020 Jun 26;40(6). doi: 10.1042/BSR20200805.
Pancreatic cancer is a kind of malignant carcinoma with high mortality, which is devoid of early diagnostic biomarker and effective therapeutic methods. Recently, long non-coding RNAs (lncRNAs) have been reported as a crucial role in regulating the development of various kinds of tumors. Here, we found lncRNA small nuclear RNA host gene 12 (SNHG12) is highly expressed in pancreatic cancer tissues and cell lines through qRT-PCR, which suggested that SNHG12 possibly accelerates the progression of pancreatic cancer. Further study revealed that SNHG12 promoted cancer cells growth and invasion via absorbing miR-320b. Flow cytometry and transwell chamber assay were utilized to verify the promoting effects on proliferation and invasion that SNHG12 acts in pancreatic cancer cells. Evidence that SNHG12 increased cell invasive ability through up-regulated EMT process was lately obtained by Western blotting assay. Consequently, we extrapolated that SNHG12/miR-320b could be invoked as a promising early diagnostic hallmark and therapeutic strategy for pancreatic cancer.
胰腺癌是一种死亡率很高的恶性癌，缺乏早期诊断的生物标志物和有效的治疗方法。最近，长非编码 RNA（lncRNA）被报道在调节各种肿瘤的发展中起着关键作用。在这里，我们通过 qRT-PCR 发现 lncRNA 小核 RNA 宿主基因 12（SNHG12）在胰腺癌组织和细胞系中高表达，这表明 SNHG12 可能加速了胰腺癌的进展。进一步的研究表明，SNHG12 通过吸收 miR-320b 促进癌细胞的生长和侵袭。流式细胞术和 Transwell 室分析用于验证 SNHG12 在胰腺癌细胞中的增殖和侵袭促进作用。Western blot 分析后来证实了 SNHG12 通过上调 EMT 过程增加细胞侵袭能力的证据。因此，我们推断 SNHG12/miR-320b 可以作为一种有前途的早期诊断标志物和胰腺癌的治疗策略。
