长链非编码 RNA ATB 通过 miR-200b/纤维连接蛋白 1 轴促进非小细胞肺癌进展。

Long non-coding RNA ATB expedites non-small cell lung cancer progression by the miR-200b/fibronectin 1 axis.

机构信息

Department of Geriatrics Medicine, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, China.

School of Medicine, Ningbo University, Ningbo, China.

出版信息

J Clin Lab Anal. 2023 Apr;37(4):e24822. doi: 10.1002/jcla.24822. Epub 2023 Feb 20.

DOI:10.1002/jcla.24822

PMID:36806318

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10020841/

Abstract

BACKGROUND

Long non-coding RNA (lncRNA) ATB belongs to an active modulator in multiple cancers, but its expression along with potential underlying non-small cell lung cancer (NSCLC) is obscure. Our study aimed to investigate the role and potential mechanism of LncRNA ATB in NSCLC.

METHODS

LncRNA ATB expression in NSCLC tissues and cell lines was detected by qRT-PCR. Effects of LncRNA ATB on NSCLC cell proliferation, migration and invasion were assessed by MTS, colony formation and transwell assays. The connection among LncRNA ATB, miR-200b and fibronectin 1 (FN1) was determined by bioformatics prediction and luciferase reporter assay.

RESULTS

In this research, upregulation of LncRNA ATB was discovered in NSCLC tissue samples and cell lines. LncRNA ATB was positively related to advanced tumor phase as well as lymph node metastasis. Cell function assays reflected LncRNA ATB expedited NSCLC cells proliferation, migration and invasion. LncRNA ATB promoted fibronectin 1 (FN1) expression via inhibiting miR-200b. Furthermore, LncRNA ATB depletion suppressed NSCLC cells proliferation, migration and invasion, while miR-200b inhibitor or pcDNA-FN1 rescued these effects.

CONCLUSION

In summary, our outcomes elucidated that LncRNA ATB/miR-200b axis expedited NSCLC cells proliferation, migration and invasion by up-regulating FN1.

摘要

背景

长链非编码 RNA（lncRNA）ATB 属于多种癌症中的一种活跃调节剂，但它在非小细胞肺癌（NSCLC）中的表达及其潜在的作用尚不清楚。本研究旨在探讨 LncRNA ATB 在 NSCLC 中的作用及其潜在机制。

方法

采用 qRT-PCR 检测 NSCLC 组织和细胞系中 LncRNA ATB 的表达。采用 MTS、集落形成和 Transwell 实验评估 LncRNA ATB 对 NSCLC 细胞增殖、迁移和侵袭的影响。通过生物信息学预测和荧光素酶报告基因实验确定 LncRNA ATB、miR-200b 和纤维连接蛋白 1（FN1）之间的关系。

结果

本研究发现 LncRNA ATB 在 NSCLC 组织样本和细胞系中上调。LncRNA ATB 与晚期肿瘤分期和淋巴结转移呈正相关。细胞功能实验表明 LncRNA ATB 促进了 NSCLC 细胞的增殖、迁移和侵袭。LncRNA ATB 通过抑制 miR-200b 促进纤维连接蛋白 1（FN1）的表达。此外，LncRNA ATB 耗竭抑制了 NSCLC 细胞的增殖、迁移和侵袭，而 miR-200b 抑制剂或 pcDNA-FN1 则挽救了这些作用。