Faculty of Engineering Sciences and Bavarian Polymer Institute, University of Bayreuth, Ludwig Thoma Str. 36A, 95447, Bayreuth, Germany.
Biofabrication. 2020 Jul 1;12(3):035027. doi: 10.1088/1758-5090/ab94cf.
In this paper, we describe the application of the 4D biofabrication approach for the fabrication of artificial nerve graft. Bilayer scaffolds consisting of uniaxially aligned polycaprolactone-poly(glycerol sebacate) (PCL-PGS) and randomly aligned methacrylated hyaluronic acid (HA-MA) fibers were fabricated using electrospinning and further used for the culture of PC-12 neuron cells. Tubular structures form instantly after immersion of fibrous bilayer in an aqueous buffer and the diameter of obtained tubes can be controlled by changing bilayer parameters such as the thickness of each layer, overall bilayer thickness, and medium counterion concentration. Designed scaffolds showed a self-folded scroll-like structure with high stability after four weeks of real-time degradation. The significance of this research is in the fabrication of tuneable tubular nerve guide conduits that can simplify the current existing clinical treatment of neural injuries.
本文介绍了 4D 生物制造方法在人工神经移植物制造中的应用。通过静电纺丝制备了由定向排列的聚己内酯-聚（癸二酸甘油酯）（PCL-PGS）和无规排列的甲基丙烯酰化透明质酸（HA-MA）纤维组成的双层支架，并进一步用于 PC-12 神经元细胞的培养。纤维双层浸入水缓冲液中后，立即形成管状结构，并且可以通过改变双层参数（例如每层的厚度、双层总厚度和介质抗衡离子浓度）来控制获得的管的直径。设计的支架在实时降解四周后显示出具有高稳定性的自折叠螺旋形结构。这项研究的意义在于制造可调管状神经引导导管，这可以简化当前神经损伤的临床治疗。
