Park Bok-Nam, Kim Jang-Hee, Lim Tae Sung, Park So Hyun, Kim Tae-Gyu, Yoon Bok Seon, Son Keoung Sun, Yoon Joon-Kee, An Young-Sil
Department of Nuclear Medicine and Molecular Imaging, School of Medicine, Ajou University, Suwon, South Korea.
Department of Pathology, School of Medicine, Ajou University, Suwon, South Korea.
Aust N Z J Psychiatry. 2020 Sep;54(9):883-891. doi: 10.1177/0004867420917467. Epub 2020 May 21.
We evaluated the effects of bone marrow-derived mesenchymal stem cells in a model of Alzheimer's disease using serial [F]Florbetaben positron emission tomography.
3xTg Alzheimer's disease mice were treated with intravenously injected bone marrow-derived mesenchymal stem cells, and animals without stem cell therapy were used as controls. Serial [F]Florbetaben positron emission tomography was performed after therapy. The standardized uptake value ratio was measured as the cortex standardized uptake value divided by the cerebellum standardized uptake value. Memory function and histological changes were observed using the Barnes maze test and β-amyloid-reactive cells.
Standardized uptake value ratio decreased significantly from day 14 after stem cell administration in the bone marrow-derived mesenchymal stem cells-treated group ( = 28). In contrast, there was no change in the ratio in control mice ( = 25) at any time point. In addition, mice that received bone marrow-derived mesenchymal stem cell therapy also exhibited significantly better memory function and less β-amyloid-immunopositive plaques compared to controls.
The therapeutic effect of intravenously injected bone marrow-derived mesenchymal stem cells in a mouse model of Alzheimer's disease was confirmed by β-amyloid positron emission tomography imaging, memory functional studies and histopathological evaluation.
我们使用连续[F]氟比他班正电子发射断层扫描技术，评估了骨髓间充质干细胞在阿尔茨海默病模型中的作用。
对3xTg阿尔茨海默病小鼠进行静脉注射骨髓间充质干细胞治疗，未接受干细胞治疗的动物作为对照。治疗后进行连续[F]氟比他班正电子发射断层扫描。标准化摄取值比率的测量方法为皮质标准化摄取值除以小脑标准化摄取值。使用巴恩斯迷宫试验和β-淀粉样蛋白反应性细胞观察记忆功能和组织学变化。
在骨髓间充质干细胞治疗组中，自干细胞给药后第14天起，标准化摄取值比率显著下降（n = 28）。相比之下，对照小鼠（n = 25）在任何时间点该比率均无变化。此外，与对照组相比，接受骨髓间充质干细胞治疗的小鼠还表现出明显更好的记忆功能和更少的β-淀粉样蛋白免疫阳性斑块。
通过β-淀粉样蛋白正电子发射断层扫描成像、记忆功能研究和组织病理学评估，证实了静脉注射骨髓间充质干细胞在阿尔茨海默病小鼠模型中的治疗效果。
