Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Pulawy, Poland.
PLoS One. 2020 May 21;15(5):e0233456. doi: 10.1371/journal.pone.0233456. eCollection 2020.
The presence of selenium in European soil is low and this causes its deficiency in livestock and, in consequence, in humans. This study aimed to obtain Lentinula (L.) edodes mycelium with the maximum content of selenium. This species was used for experiment based on its documented medicinal properties. Calves were fed with selenium-enriched L. edodes mycelium, and serum selenium concentration, average daily weight gains and selected immune parameters were estimated. The selenium-enriched mushroom was found to be safe based on cytotoxicity tests (MTT and LDH tests) and for this reason it was used for further experiments. The mean quantity of selenium in the serum of calves fed with selenium-enriched L. edodes mycelium was significantly higher than that of control calves. Additionally, the calves fed with selenium-enriched L. edodes mycelium had higher body weight gains than those of control calves. White blood cell counts and subpopulations of lymphocytes in the experimental and control calves were within the reference range. The administration of L. edodes enriched with selenium had a beneficial effect on state of health of the calves.
欧洲土壤中硒的含量较低，这导致牲畜硒缺乏，进而导致人类硒缺乏。本研究旨在获得具有最大硒含量的香菇菌丝体。基于香菇已被证实的药用特性，选择该物种进行实验。给小牛喂食富硒香菇菌丝体，并评估血清硒浓度、平均日增重和选定的免疫参数。基于细胞毒性试验（MTT 和 LDH 试验），富硒蘑菇被认为是安全的，因此将其用于进一步的实验。与对照组小牛相比，喂食富硒香菇菌丝体的小牛血清中的硒含量显著更高。此外，喂食富硒香菇菌丝体的小牛体重增加量高于对照组小牛。实验组和对照组小牛的白细胞计数和淋巴细胞亚群均在参考范围内。用富硒香菇进行的给药对小牛的健康状况有有益影响。
