Department of Pharmaceutical Botany Jagiellonian, Faculty of Pharmacy, University Medical College, 9 Medyczna Street, 30-688 Kraków, Poland.
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688 Kraków, Poland.
Molecules. 2020 Dec 31;26(1):162. doi: 10.3390/molecules26010162.
Worldwide, mushrooms belonging to the spp. such as , , and are highly valued not only for their taste and aroma but also for their health-promoting properties. These species are rich in bioelements, vitamins, and above all, compounds that exhibit immunostimulatory activity. Therefore, in this study, we aimed to determine the effect of the supplementation of culture media using inorganic Mg and Zn salts. This is the first study to establish the bioavailability of the selected elements (Mg and Zn) and anions (Cl, SO) from the enriched biomass by means of the extraction of lyophilized mycelium into artificial digestive juices. The following salts were added to the liquid Oddoux medium: ZnSO·7HO, ZnCl, MgSO·7HO or MgCl·6HO. The bioelements, anions and organic compounds in the obtained biomass were determined. The addition of Zn and Mg salts to the media increased the production of biomass by 30% and increased the bioaccumulation of the inorganic salts. Maintaining in vitro cultures under optimized and controlled conditions produced mycelium with a better composition and health properties than otherwise. Such enriched biomass may be classified as potential functional foods, aiding in overcoming deficiencies of elements and organic compounds with biological activity in humans.
在全球范围内, spp. 属的蘑菇,如 、 和 ,不仅因其味道和香气而受到高度重视,还因其具有促进健康的特性。这些物种富含生物元素、维生素,尤其是具有免疫刺激活性的化合物。因此,在这项研究中,我们旨在确定使用无机 Mg 和 Zn 盐补充培养基的效果。这是首次研究从富生物质中通过冻干菌丝体提取到人工消化液来确定选定元素(Mg 和 Zn)和阴离子(Cl、SO )的生物利用度。将以下盐添加到液体 Oddoux 培养基中:ZnSO·7HO、ZnCl、MgSO·7HO 或 MgCl·6HO。测定了获得的生物质中的生物元素、阴离子和有机化合物。向培养基中添加 Zn 和 Mg 盐可将生物量的产量提高 30%,并增加无机盐的生物积累。在优化和控制条件下进行体外培养可产生具有更好成分和健康特性的菌丝体,优于其他条件。这种富生物质可被归类为潜在的功能性食品,有助于克服人类元素和具有生物活性的有机化合物的缺乏。