Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Center for Implantable Devices, Purdue University, West Lafayette, Indiana 47907, United States.
School of Engineering Technology, Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, United States.
ACS Appl Mater Interfaces. 2020 Jun 17;12(24):26893-26904. doi: 10.1021/acsami.0c04729. Epub 2020 Jun 4.
Here we demonstrate a simple and scalable nanotexturing method for both planar (films) and nonplanar (tubes) polytetrafluoroethylene (PTFE) surfaces using a commercial desktop oxygen plasma etcher. The simple process can generate semiordered nanopillar structures on both tubular and planar samples with high radial and axial uniformity. We found that the resulting surfaces exhibit good bactericidal and anti-inflammatory properties. When tested against , the nanotextured surfaces showed significantly decreased live bacteria coverage and increased dead bacteria coverage, demonstrating significant bactericidal functionality. Moreover, the etched planar PTFE films exhibited better healing and inflammatory responses in the subcutis of C57BL/6 mice over 7 and 21 days, evidenced by a thinner inflammatory band, lower collagen deposition, and decreased macrophage infiltration. Our results suggest the possibility of using this simple process to generate large scale biomimetic nanotextured surfaces with good antibiofouling properties to enhance the functionality of many implantable and other biomedical devices.
在这里，我们展示了一种使用商业台式氧气等离子体蚀刻机对平面（薄膜）和非平面（管）聚四氟乙烯（PTFE）表面进行简单且可扩展的纳米结构化方法。该简单的工艺可以在管状和平面样品上生成具有高径向和轴向均匀性的半有序纳米柱结构。我们发现，所得表面具有良好的杀菌和抗炎性能。在针对金黄色葡萄球菌的测试中，纳米结构化表面显示出活细菌覆盖率显著降低和死细菌覆盖率增加，表现出显著的杀菌功能。此外，在 C57BL/6 小鼠的皮下组织中，经过蚀刻的平面 PTFE 薄膜在 7 天和 21 天内表现出更好的愈合和炎症反应，表现为炎症带更薄、胶原蛋白沉积减少和巨噬细胞浸润减少。我们的结果表明，使用这种简单的工艺生成具有良好抗生物污染性能的大规模仿生纳米结构化表面的可能性，以增强许多可植入和其他生物医学设备的功能。
