Faculdade Dinâmica Do Vale Do Ipiranga, Bairro Paraíso, Ponte Nova, 35430302, Minas Gerais, Brazil.
Laboratório de Biofármacos, Universidade Federal de Viçosa (UFV), Vila Giannet N°24, Viçosa, 36571-000, Minas Gerais, Brazil.
J Ethnopharmacol. 2020 Oct 5;260:112898. doi: 10.1016/j.jep.2020.112898. Epub 2020 May 11.
Equisetum giganteum has been traditionally used as an anti-diabetic herbal remedy to treat diabetes in the southern state of Rio Grande do Sul in Brazil.
Considering the ethonopharmacology and historical importance of E. giganteum, its potential antidiabetic effect was evaluated in alloxan induced diabetic rabbits.
Samples of Equisetum giganteum were collected in the city of Ouro Preto, Minas Gerais, Brazil. Butanolic and aqueous extracts were prepared and subsequently evaluated for anti-diabetic properties in vivo using albino male rabbits. At the end of the treatment period, the animals were euthanized, and histopathological analysis were carried out. The following biochemical parameters were studied: glucose, triacylglycerol, cholesterol, albumin, creatinine, glycosylated hemoglobin and lipase. The phytochemical profile of the extracts was studied by liquid chromatography techniques coupled to a UV/VIS detector and high-resolution mass spectrometry.
Both aqueous and butanolic extracts were capable of reducing significantly the levels of glucose, cholesterol and triacylglycerol and thus demonstrating their hypolipidemic and hypoglycemiant effects. Furthermore, the extracts prevented the occurrence of hepatic complications during treatment. The phytochemical profile of the extracts was investigated, and the natural products detected were in agreement with those that had been previously described in the literature.
Based on the significant reductions in biochemical parameters and the histologic evidence for the absence of complications in the liver, pancreas of the treated animals, Equisetum giganteum can be a therapeutically relevant resource in the treatment of diabetes and hyperlipidemia.
在巴西南里奥格兰德州，木贼一直被传统用作抗糖尿病草药，用于治疗糖尿病。
考虑到木贼的民族药理学和历史重要性，评估其在四氧嘧啶诱导的糖尿病兔中的潜在抗糖尿病作用。
在巴西米纳斯吉拉斯州的欧鲁普雷图市采集木贼样品。制备丁醇和水提取物，并随后在体内使用白化雄性兔评估其抗糖尿病特性。在治疗期末，处死动物并进行组织病理学分析。研究了以下生化参数：葡萄糖、三酰甘油、胆固醇、白蛋白、肌酐、糖化血红蛋白和脂肪酶。通过液相色谱技术与紫外/可见检测器和高分辨率质谱联用研究了提取物的植物化学特征。
水提物和丁醇提物均能显著降低葡萄糖、胆固醇和三酰甘油水平，从而显示其降血脂和降血糖作用。此外，提取物可预防治疗期间肝并发症的发生。研究了提取物的植物化学特征，并检测到的天然产物与文献中先前描述的一致。
基于生化参数的显著降低以及肝脏、胰腺组织学证据无并发症，木贼可作为治疗糖尿病和高脂血症的有治疗相关性的资源。
