R&D Center, Naturetech Co., Ltd., 450-86, Maebong-Ro, Dongnam-Gu, Cheonan-Si, Chungnam, 31257, South Korea.
Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672, Yuseong-Daero, Yuseong-Gu, Daejeon, 34054, South Korea.
J Ethnopharmacol. 2020 Oct 5;260:112985. doi: 10.1016/j.jep.2020.112985. Epub 2020 May 18.
Alpinia Oxyphylla Miquel is a widely used traditional herbal medicine for the treatment of abdominal pain, intestinal disorders, enuresis, and inflammatory conditions.
In this study we aimed to demonstrate the anti-inflammatory and anti-nociceptive effects of Alpinia Oxyphylla Miquel extracts with 50% ethanol (AOE) in animal models.
The anti-inflammatory and nociceptive effects of AOE were estimated in vivo using a carrageenan-induced rat paw edema model, an arachidonic acid-induced mice ear edema model, an acetic acid induced mice writhing test model, and an ex vivo sulfated glycosaminoglycan (GAG) inhibition assay. In vitro inhibition assays for cyclooxygenase (COX) and lipoxygenase (LOX) were performed to determine the IC of AOE.
Compared with the control, AOE caused a significant inhibition of paw edema, ear thickness, and abdominal constriction in animals. Compared with untreated explants exposed to interleukin-l alpha (IL-1α), preoteoglycan degradation by IL-1α was inhibited (18.2%-82.0%) in explants treated with AOE (50-400 μg/ml). In vitro, IC values of AOE were 299.87, 199.07, and 170.29 μg/ml for COX-1, COX-2, and 5-LOX respectively.
These data demonstrate the potent anti-inflammatory and analgesic effects of AOE and suggest AOE to be useful for the treatment of osteoarthritis and/or associated symptoms.
益智是一种广泛应用于传统草药医学的草药，用于治疗腹痛、肠道疾病、遗尿和炎症性疾病。
本研究旨在通过动物模型证明益智 50%乙醇提取物（AOE）的抗炎和镇痛作用。
使用角叉菜胶诱导的大鼠足肿胀模型、花生四烯酸诱导的小鼠耳肿胀模型、醋酸诱导的小鼠扭体试验模型和体外硫酸化糖胺聚糖（GAG）抑制试验，评估 AOE 的抗炎和镇痛作用。通过环加氧酶（COX）和脂加氧酶（LOX）的体外抑制试验测定 AOE 的 IC。
与对照组相比，AOE 显著抑制了动物的足肿胀、耳厚度和腹部收缩。与未处理的对照软骨外植体相比，在暴露于白细胞介素-1α（IL-1α）的软骨外植体中，IL-1α 诱导的蛋白聚糖降解被 AOE（50-400μg/ml）抑制（18.2%-82.0%）。体外，AOE 对 COX-1、COX-2 和 5-LOX 的 IC 值分别为 299.87、199.07 和 170.29μg/ml。
这些数据表明 AOE 具有强大的抗炎和镇痛作用，并提示 AOE 可用于治疗骨关节炎和/或相关症状。
