Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, United Kingdom.
RNA. 2020 Sep;26(9):1081-1085. doi: 10.1261/rna.075895.120. Epub 2020 May 21.
We reported previously that, in budding yeast, transcription rate affects both the efficiency and fidelity of pre-mRNA splicing, especially of ribosomal protein transcripts. Here, we report that the majority of ribosomal protein transcripts with non-consensus 5' splice sites are spliced less efficiently when transcription is faster, and more efficiently with slower transcription. These results support the "window of opportunity" model, and we suggest a possible mechanism to explain these findings.
我们之前曾报道过，在出芽酵母中，转录速率会影响前体 mRNA 剪接的效率和保真度，尤其是核糖体蛋白转录本。在这里，我们报告说，当转录速度较快时，大多数具有非共识 5'剪接位点的核糖体蛋白转录本的剪接效率较低，而当转录速度较慢时，剪接效率较高。这些结果支持“机会之窗”模型，并且我们提出了一种可能的机制来解释这些发现。
