State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Chemistry and Chemical Engineering, Tiangong University, Tianjin, 300387, China.
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Chemistry and Chemical Engineering, Tiangong University, Tianjin, 300387, China.
Carbohydr Res. 2020 Jul;493:108030. doi: 10.1016/j.carres.2020.108030. Epub 2020 May 12.
Bacterial cellulose(BC) is a kind of extracellular polymer synthesized by bacteria and it has very wide applications in many fields. However, the application of BC in a large commercial scale can still not be fulfilled due to the low yield and demanding for BC membranes with very different properties. To this end, a new BC-producer Komagataeibacter rhaeticus TJPU03 was isolated from rotten orange peel, which produced 8.28 ± 0.27 g/L(dry weight) in standard HS medium at the 10th day. The membrane is easier to be purified by one-step alkaline treatment and the produced BC(K-BC) membranes possess homogeneous, looser and more porous three-dimensional network composed by thinner cellulose fibrils. However, the wet K-BC possesses stronger mechanical properties and exhibits lower toxicity and higher cytocompatibility to mammalian cell. Owing to the more porous and homogeneous network, K-BC possesses high loading capacity of cell and protein drugs. Also, it exhibits sustained-controlled release ability for proteinaceous drug. The high yield of this strain and the special characteristics of K-BC predict this strain to be a very promising BC-producer and broad applications of K-BC in the fields of wound healing, scaffolds of tissue engineering, tissue repair and regeneration.
细菌纤维素（BC）是一种由细菌合成的细胞外聚合物，它在许多领域都有非常广泛的应用。然而，由于 BC 的产量低，而且对性质非常不同的 BC 膜的需求很高，因此其在大规模商业应用中仍然无法实现。为此，从腐烂的桔皮中分离出一种新的 BC 生产菌Komagataeibacter rhaeticus TJPU03，在标准 HS 培养基中第 10 天可生产 8.28±0.27g/L（干重）。该膜更容易通过一步碱处理进行纯化，所生产的 BC（K-BC）膜具有由更薄的纤维素纤维组成的均匀、更疏松和更多孔的三维网络。然而，湿 K-BC 具有更强的机械性能，对哺乳动物细胞表现出更低的毒性和更高的细胞相容性。由于具有更多孔和均匀的网络，K-BC 具有较高的细胞和蛋白药物载药量。同时，它对蛋白质药物表现出持续控制释放能力。该菌株的高产量和 K-BC 的特殊特性预示着该菌株将成为一种非常有前途的 BC 生产菌，K-BC 将在伤口愈合、组织工程支架、组织修复和再生等领域得到广泛应用。
