Tseng Yi Sheng, Patel Anil Kumar, Chen Chiu-Wen, Dong Cheng-Di, Singhania Reeta Rani
Institute of Aquatic Science and Technology, National Kaohsiung University of Science and Technology, Kaohsiung City, 81157 Taiwan.
Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, 81157 Taiwan.
J Food Sci Technol. 2023 Mar;60(3):1054-1064. doi: 10.1007/s13197-022-05451-y. Epub 2022 Jul 20.
Bacterial cellulose (BC) has attracted worldwide attention owing to its tremendous properties and versatile applications. BC has huge market demand, however; its production is still limited hence important to explore the economically and technically feasible bioprocess for its improved production. The current study is based on improving the bioprocess for BC production employing 14148. Physico-chemical parameters have been optimized initial pH, incubation temperature, incubation period, inoculum size, and carbon source for maximum BC production. The study employed crude and/or a defined carbon source in the production medium. Hestrin and Schramm (HS) medium was used for BC production with initial pH 5.5 at 30 °C after 7 days of incubation under static conditions. The yield of BC obtained from fruit juice extracted from orange, papaya, mango and banana were higher than other sugars employed. The maximum BC yield of 3.48 ± 0.16 g/L was obtained with papaya extract having 40 g/L reducing sugar concentration and 3.47 ± 0.05 g/L BC was obtained with orange extract having 40 g/L reducing sugar equivalent in the medium. BC yield was about three-fold higher than standard HS medium. Fruit extracts can be employed as sustainable and economic substrates for BC production to replace glucose and fructose.
The online version contains supplementary material available at 10.1007/s13197-022-05451-y.
细菌纤维素（BC）因其优异的性能和广泛的应用而受到全球关注。然而，BC市场需求巨大，其产量仍然有限，因此探索经济和技术上可行的生物工艺以提高其产量具有重要意义。当前的研究基于改进使用14148生产BC的生物工艺。对初始pH、培养温度、培养时间、接种量和碳源等物理化学参数进行了优化，以实现BC的最大产量。该研究在生产培养基中使用了粗碳源和/或特定碳源。在静态条件下培养7天后，使用赫斯特林和施拉姆（HS）培养基在30℃、初始pH 5.5的条件下生产BC。从橙子、木瓜、芒果和香蕉中提取的果汁获得的BC产量高于其他使用的糖类。在培养基中还原糖浓度为40 g/L的木瓜提取物获得了最高BC产量3.48±0.16 g/L，还原糖当量为40 g/L的橙子提取物获得了3.47±0.05 g/L的BC产量。BC产量比标准HS培养基高出约三倍。水果提取物可作为可持续且经济的底物用于BC生产，以替代葡萄糖和果糖。
在线版本包含可在10.1007/s13197-022-05451-y获取的补充材料。
