Université de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD, Nantes, France.
Institut des Maladies de l'Appareil Digestif, IMAD, Hôtel Dieu, CHU Nantes, Nantes, France.
Gut. 2021 Jan;70(1):6-8. doi: 10.1136/gutjnl-2020-321228. Epub 2020 May 23.
Full-field optical coherence tomography (FFOCT) is an imaging technique of biological tissue based on tissue light reflectance analysis. We evaluated the feasibility of imaging fresh digestive mucosal biopsies after a quick mounting procedure (5 min) using two distinct modalities of FFOCT. In static FFOCT mode, we gained high-resolution images of general gut tissue-specific architecture, such as oesophageal papillae, gastric pits, duodenal villi and colonic crypts. In dynamic FFOCT mode, we imaged individual epithelial cells of the mucosal lining with a cellular or subcellular resolution and identified cellular components of the lamina propria. FFOCT represents a promising dye-free imaging tool for on-site analysis of gut tissue remodelling.
全场光学相干断层扫描（FFOCT）是一种基于组织光反射分析的生物组织成像技术。我们评估了使用两种不同的 FFOCT 模式对经过快速安装程序（5 分钟）后的新鲜消化黏膜活检进行成像的可行性。在静态 FFOCT 模式下，我们获得了食管乳头、胃小凹、十二指肠绒毛和结肠隐窝等一般肠道组织特定结构的高分辨率图像。在动态 FFOCT 模式下，我们以细胞或亚细胞分辨率对黏膜衬里的单个上皮细胞进行成像，并识别固有层的细胞成分。FFOCT 代表了一种有前途的无染料成像工具，可用于现场分析肠道组织重塑。
