CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India.
Arch Microbiol. 2020 Sep;202(7):1839-1848. doi: 10.1007/s00203-020-01905-z. Epub 2020 May 23.
As a part of studying the effect of deoxygenation, eutrophication and acidification on bacterial diversity, strain HWN-4 was isolated from tube well water and characterized. The draft genome sequencing of strain HWN-4 revealed a genome size of 5,774,764 bp and the annotation indicated 5102 coding sequences including 66 RNA genes. Strain HWN-4 is Gram negative, rod-shaped, motile in the log phase, catalase and oxidase positive, and the major fatty acids and respiratory quinone present are C 3-OH, C 3OH/C iso I, C ω7c/C ω6c, C and C cyclo and ubiquinone-8, respectively. The phylogenetic analyses, based on 16S rRNA gene sequence, indicated that strain HWN-4 is a member of the genus Mitsuaria. The average nucleotide identity (ANI) and genome-to-genome similarity between strain HWN-4 and all other species/strains of the genus Mitsuaria are less than (%) 95.0 and 70.0, respectively. This confirms the status of strain HWN-4 as a novel species. The species status is further confirmed by phenotypic differences exhibited by strain HWN-4 with other members of the same genus. Based on the collective differences exhibited by strain HWN-4 with other members of the genus Mitsuaria, the name Mitsuaria chitinivorans sp. nov. is proposed. Further, the diagnostic signature nucleotides were identified in the 16S rRNA gene sequences of members of the genera Mitsuaria, Pelomonas and Roseateles, that distinctly differentiate them and support an emendation of the genera. Besides, phylogenetic and structural characterization of chitinases from members of the genus Mitsuaria was performed. The type strain of Mitsuaria chitinivorans sp. nov. is HWN-4 = LMG 28685 = KTCC 42483.
作为研究脱氧、富营养化和酸化对细菌多样性影响的一部分，从管井水中分离出菌株 HWN-4 并对其进行了特征描述。菌株 HWN-4 的全基因组测序结果表明，其基因组大小为 5774764bp，注释显示包含 66 个 RNA 基因在内的 5102 个编码序列。菌株 HWN-4 呈革兰氏阴性、杆状、对数生长期运动、过氧化氢酶和氧化酶阳性，主要脂肪酸和呼吸醌分别为 C3-OH、C3OH/CisoI、Cω7c/Cω6c、C 和 C 环以及 ubiquinone-8。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HWN-4 是 Mitsuaria 属的一个成员。与 Mitsuaria 属的所有其他种/菌株的平均核苷酸同一性 (ANI) 和基因组到基因组相似度均小于 95.0%和 70.0%，这证实了菌株 HWN-4 是一个新种的地位。菌株 HWN-4 与同属其他成员表现出的表型差异进一步证实了其种的地位。基于菌株 HWN-4 与 Mitsuaria 属其他成员表现出的综合差异，提出了 Mitsuaria chitinivorans sp. nov.的新种名。此外，还鉴定了 Mitsuaria、Pelomonas 和 Roseateles 属成员 16S rRNA 基因序列中的诊断特征核苷酸，这些特征核苷酸明显地区分了它们，并支持对这些属的修正。此外，还对 Mitsuaria 属成员的几丁质酶进行了系统发育和结构特征分析。Mitsuaria chitinivorans sp. nov.的模式菌株为 HWN-4=LMG 28685=KTCC 42483。
