National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Fengxian District, Shanghai 201403, China.
National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Fengxian District, Shanghai 201403, China; College of Life Science, Nanjing Agricultural University, No.1, Weigang road, XuanWu District, Nanjing 210095, China.
Fungal Biol. 2020 Jun;124(6):551-561. doi: 10.1016/j.funbio.2020.02.010. Epub 2020 Feb 27.
Hypsizygus marmoreus is an important commercial edible fungus, but the lack of basic studies on this fungus has hindered further development of its commercial value. In this study, we found that the treatment of damaged vegetative mycelia with 1 mM l-ascorbic acid (ASA) significantly increased the antioxidant enzyme activities (GPX, GR, CAT and SOD) and antioxidant contents (GSH and ASA) and reduced the ROS levels (HO and O) in mechanically damaged mycelia. Additionally, this treatment increased mycelial biomass. At the reproductive stage, our results demonstrated that the treatment of damaged H. marmoreus mycelia with 2.24 mM ASA significantly increased the antioxidant enzyme activities (GPX, GR, GST, TRXR and CAT), endogenous ASA contents and GSH/GSSG ratios in different developmental stages and significantly decreased the MDA and HO contents. Furthermore, this study showed that the expression levels of the antioxidant enzyme genes were consistent with the enzyme activities. Damaged mycelia treated with ASA regenerated 2-3 d earlier than the control group and showed significantly enhanced fruiting body production. These results suggested that exogenous ASA regulated mycelia intracellular ASA content to increase mycelial antioxidant abilities, induce the regeneration of damaged mycelia and regulate the development of fruiting bodies in H. marmoreus.
血红密环菌是一种重要的商业食用真菌，但由于对该真菌缺乏基础研究，限制了其商业价值的进一步开发。在本研究中，我们发现用 1mM l-抗坏血酸（ASA）处理受损的营养菌丝可以显著提高抗氧化酶活性（GPX、GR、CAT 和 SOD）和抗氧化含量（GSH 和 ASA），降低ROS 水平（HO 和 O）。此外，这种处理还可以增加菌丝体的生物量。在生殖阶段，我们的结果表明，用 2.24mM ASA 处理受损的血红密环菌菌丝可以显著提高不同发育阶段的抗氧化酶活性（GPX、GR、GST、TRXR 和 CAT）、内源性 ASA 含量和 GSH/GSSG 比值，显著降低 MDA 和 HO 的含量。此外，本研究表明，抗氧化酶基因的表达水平与酶活性一致。用 ASA 处理的受损菌丝比对照组早 2-3 天再生，并显著提高了子实体的产量。这些结果表明，外源 ASA 调节菌丝细胞内 ASA 含量，提高菌丝体的抗氧化能力，诱导受损菌丝的再生，并调节血红密环菌子实体的发育。
