National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 309 Room, No. 1000, Jinqi Road, Fengxian District, Shanghai, 201403, China.
College of Life Science, Nanjing Agricultural University, No. 1, Weigang road, XuanWu District, Nanjing, 210095, China.
Appl Microbiol Biotechnol. 2020 Dec;104(24):10555-10570. doi: 10.1007/s00253-020-10981-6. Epub 2020 Nov 4.
Glutathione peroxidase (GPX) is one of the most important antioxidant enzymes for maintaining reactive oxygen species (ROS) homeostasis. Although studies on fungi have suggested many important physiological functions of GPX, few studies have examined the role of this enzyme in Basidiomycetes, particularly its functions in fruiting body developmental processes. In the present study, GPX-silenced (GPxi) strains were obtained by using RNA interference. The GPxi strains of Hypsizygus marmoreus showed defects in mycelial growth and fruiting body development. In addition, the results indicated essential roles of GPX in controlling ROS homeostasis by regulating intracellular HO levels, maintaining GSH/GSSG balance, and promoting antioxidant enzyme activity. Furthermore, lignocellulose enzyme activity levels were reduced and the mitochondrial phenotype and mitochondrial complex activity levels were changed in the H. marmoreus GPxi strains, possibly in response to impediments to mycelial growth and fruiting body development. These findings indicate that ROS homeostasis has a complex influence on growth, fruiting body development, GSH/GSSG balance, and carbon metabolism in H. marmoreus.Key points• ROS balance, energy metabolism, fruiting development.
谷胱甘肽过氧化物酶（GPX）是维持活性氧（ROS）平衡的最重要的抗氧化酶之一。尽管真菌研究表明 GPX 具有许多重要的生理功能，但很少有研究探讨该酶在担子菌中的作用，特别是其在子实体发育过程中的功能。在本研究中，通过 RNA 干扰获得了谷胱甘肽过氧化物酶沉默（GPxi）菌株。GPxi 菌株的糙皮侧耳菌丝体生长和子实体发育出现缺陷。此外，结果表明 GPX 通过调节细胞内 HO 水平、维持 GSH/GSSG 平衡和促进抗氧化酶活性，在控制 ROS 平衡中起重要作用。此外，糙皮侧耳 GPxi 菌株的木质纤维素酶活性水平降低，线粒体表型和线粒体复合物活性水平发生变化，这可能是由于菌丝体生长和子实体发育受阻所致。这些发现表明，ROS 平衡对子实体生长、发育、GSH/GSSG 平衡和碳代谢有复杂的影响。
ROS 平衡、能量代谢、子实体发育。
