Zhang Jinjing, Hao Haibo, Liu Hong, Wang Qian, Chen Mingjie, Feng Zhiyong, Chen Hui
National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Shanghai, 201403, China.
College of Life Science, Nanjing Agricultural University, No. 1, Weigang Road, Xuanwu District, Nanjing, 210095, China.
Appl Microbiol Biotechnol. 2021 Apr;105(7):2815-2829. doi: 10.1007/s00253-021-11175-4. Epub 2021 Mar 6.
Zn(II)Cys transcription factors are critical for the reproductive growth and sexual development of fungi, but their roles in Basidiomycota remain unclear. In this study, the Hypsizygus marmoreus gene hada-1 was shown to encode a Zn(II)Cys transcription factor, the growth rate of mycelia was decreased, hyphae were angulated, and fruiting body development was hindered in the hada-1-silenced strains. In addition, mitochondrial stability was lost, and the mitochondria morphologies changed from oval shaped to dumbbell or linear shaped in the silenced strains. Regarding mitochondrial instability, the mitochondrial complex II, III, and V activities and adenosine triphosphate content were significantly decreased. At the same time, the activities of the carbohydrate metabolism-related enzymes glucose-6-plosphatase, glucose dehydrogenase, and laccase were significantly decreased, which might have resulted in the reduction of carbon metabolism. Furthermore, hada-1 was shown to regulate the reactive oxygen species (ROS) level; compared with the wild-type (WT) strain, the silenced mycelia exhibited higher ROS contents and were more sensitive to oxidative stress. Taken together, these results indicate that, as a global regulator, hada-1 plays crucial roles in mycelial growth, fruiting body development, carbon metabolism, mitochondrial stability, and oxidative stress in the basidiomycete H. marmoreus. KEY POINTS: • Zn(II)Cys transcription factor, mitochondrial stability, fruiting body development.
锌（II）半胱氨酸转录因子对真菌的生殖生长和有性发育至关重要，但其在担子菌中的作用仍不清楚。在本研究中，滑菇基因hada-1被证明编码一种锌（II）半胱氨酸转录因子，在hada-1沉默菌株中，菌丝体生长速率降低，菌丝呈角状，子实体发育受阻。此外，沉默菌株中线粒体稳定性丧失，线粒体形态从椭圆形变为哑铃形或线形。关于线粒体不稳定性，线粒体复合物II、III和V的活性以及三磷酸腺苷含量显著降低。同时，碳水化合物代谢相关酶葡萄糖-6-磷酸酶、葡萄糖脱氢酶和漆酶的活性显著降低，这可能导致碳代谢减少。此外，hada-1被证明可调节活性氧（ROS）水平；与野生型（WT）菌株相比，沉默菌丝体表现出更高的ROS含量，并且对氧化应激更敏感。综上所述，这些结果表明，作为一种全局调节因子，hada-1在担子菌滑菇的菌丝体生长、子实体发育、碳代谢、线粒体稳定性和氧化应激中起关键作用。要点：•锌（II）半胱氨酸转录因子、线粒体稳定性、子实体发育。
