通过单细胞凝胶电泳（彗星试验）评估二甲戊灵对中国仓鼠卵巢细胞的遗传毒性作用。
Assessment of Genotoxic Effects of Pendimethalin in Chinese Hamster Over Cells by the Single Cell Gel Electrophoresis (Comet) Assay.
作者信息
Demir Nazlı, Aydin Sevtap, Ündeğer Bucurgat Ülkü
机构信息
Hacettepe University, Faculty Of Pharmacy, Department Of Pharmaceutical Toxicology, Ankara, Turkey.
出版信息
Turk J Pharm Sci. 2017 Aug;14(2):185-190. doi: 10.4274/tjps.79663. Epub 2017 Aug 15.
OBJECTIVES
Pendimethalin (-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzeneamine) is a dinitroaniline herbicide compound which selectively controls weeds. It is a cell division and growth inhibitor. It descends plants in a short time after seedling. It is a soil and water pollutant due to the widespread use of formulations in Turkey and around the world. Pendimethalin is manufactured in and imported by Turkey. Pendimethalin is a slightly toxic compound that is classified in toxicity class 3 by the United States Environmental Protection Agency (USEPA). Even though it is classified as group C (human possible carcinogen) compound by the USEPA, there are limited number of studies about its genotoxic effects. The aim of this study was to evaluate genotoxic effects of different concentrations of pendimethalin in Chinese hamster over (CHO) cells by the single cell gel electrophoresis (comet) assay.
MATERIALS AND METHODS
The cells are incubated with 1, 10, 100, 1000 and 10000 µM concentrations of pendimethalin for 30 min at 37°C and DNA damage was compared with CHO cells untreated with pendimethalin. 50 µM hydrogen peroxide was used as positive control.
RESULTS
No significant cytotoxic effects were observed within the concentration ranges studied. The DNA damage in CHO cells was significantly increased in the pendimethalin concentrations of 1, 100, 1000 and 10000 µM, however, a significant decrease was observed in 10 µM pendimethalin concentration.
CONCLUSION
Our results show that 1-10000 µM concentrations of pendimethalin induce DNA damage in CHO cells, which was assessed by comet assay.
目的
二甲戊灵（-（1-乙基丙基）-3,4-二甲基-2,6-二硝基苯胺）是一种二硝基苯胺类除草剂化合物，可选择性地控制杂草。它是一种细胞分裂和生长抑制剂。它在幼苗后短时间内使植物枯萎。由于其制剂在土耳其和世界各地广泛使用，它是一种土壤和水污染物质。二甲戊灵在土耳其生产和进口。二甲戊灵是一种微毒化合物，被美国环境保护局（USEPA）归类为毒性3类。尽管它被USEPA归类为C组（人类可能致癌物）化合物，但关于其遗传毒性作用的研究数量有限。本研究的目的是通过单细胞凝胶电泳（彗星）试验评估不同浓度的二甲戊灵对中国仓鼠卵巢（CHO）细胞的遗传毒性作用。
材料和方法
将细胞与1、10、100、1000和10000μM浓度的二甲戊灵在37°C下孵育30分钟，并将DNA损伤与未用二甲戊灵处理的CHO细胞进行比较。50μM过氧化氢用作阳性对照。
结果
在所研究的浓度范围内未观察到明显的细胞毒性作用。在1、100、1000和10000μM的二甲戊灵浓度下，CHO细胞中的DNA损伤显著增加，然而，在10μM二甲戊灵浓度下观察到显著降低。
结论
我们的结果表明，通过彗星试验评估，1-10000μM浓度的二甲戊灵可诱导CHO细胞中的DNA损伤。
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