（爵床科植物）九头狮子草正己烷提取物的抗疟活性
Antiplasmodial Activity of the -Hexane Extract from (Jacq. ex. Fr) P. Kumm.
作者信息
Afieroho Ozadheoghene Eriarie, Noundou Xavier Siwe, Onyia Chiazor P, Festus Osamuyi H, Chukwu Elizabeth C, Adedokun Olutayo M, Isaacs Michelle, Hoppe Heinrich C, Krause Rui Wm, Abo Kio A
机构信息
University of Port Harcourt, Faculty of Pharmaceutical Sciences, Department of Pharmacognosy and Phytotherapy, Port Harcourt, Nigeria.
Rhodes University, Faculty of Science, Department of Chemistry, Grahamstown, South Africa.
出版信息
Turk J Pharm Sci. 2019 Mar;16(1):37-42. doi: 10.4274/tjps.18894. Epub 2018 Dec 31.
OBJECTIVES
Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom and the isolation of a sterol from the extract.
MATERIALS AND METHODS
Antiplasmodial and cytotoxicity assays were done using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the -hexane extract was elucidated using spectroscopic techniques.
RESULTS
The -hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC=0.016 μg/mL) > -hexane extract (IC=25.18 μg/mL). It also showed mild cytotoxicity (IC>100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC=0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract.
CONCLUSION
This study reporting for the first time the antiplasmodial activity of revealed its nutraceutical potential in the management of malaria.
目的
据报道，有几种蘑菇具有捕食线虫和抗原生动物的特性。本研究报告了食用蘑菇正己烷提取物的抗疟原虫和细胞毒性特性，以及从该提取物中分离出一种甾醇。
材料与方法
分别使用疟原虫乳酸脱氢酶测定法和人宫颈癌HeLa细胞系进行抗疟原虫和细胞毒性测定。采用光谱技术阐明了从正己烷提取物中分离出的化合物的结构。
结果
正己烷提取物（得率：0.93% w/w）表现出剂量依赖性抗疟原虫活性，其对寄生虫的抑制趋势为：氯喹（IC = 0.016 μg/mL）>正己烷提取物（IC = 25.18 μg/mL）。与参比药物吐根碱（IC = 0.013 μg/mL）相比，它还表现出轻度细胞毒性（IC>100 μg/mL；选择性指数>4）。从提取物中分离并鉴定出了已知的甾醇，即麦角甾-5,7,22-三烯-3-醇。
结论
本研究首次报道了[具体蘑菇名称未给出]的抗疟原虫活性，揭示了其在疟疾管理中的营养保健潜力。
Zotero 插件