Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Molecules. 2011 Jan 14;16(1):583-9. doi: 10.3390/molecules16010583.
The methanol and fractionated extracts (hexane, ethyl acetate and water) of Alpinia mutica (Zingiberaceae) rhizomes were investigated for their cytotoxic effect against six human carcinoma cell lines, namely KB, MCF7, A549, Caski, HCT116, HT29 and non-human fibroblast cell line (MRC 5) using an in vitro cytotoxicity assay. The ethyl acetate extract possessed high inhibitory effect against KB, MCF7 and Caski cells (IC₅₀ values of 9.4, 19.7 and 19.8 µg/mL, respectively). Flavokawin B (1), 5,6-dehydrokawain (2), pinostrobin chalcone (3) and alpinetin (4), isolated from the active ethyl acetate extract were also evaluated for their cytotoxic activity. Of these, pinostrobin chalcone (3) and alpinetin (4) were isolated from this plant for the first time. Pinostrobin chalcone (3) displayed very remarkable cytotoxic activity against the tested human cancer cells, such as KB, MCF7 and Caski cells (IC₅₀ values of 6.2, 7.3 and 7.7 µg/mL, respectively). This is the first report of the cytotoxic activity of Alpinia mutica.
采用体外细胞毒性试验，考察了益智（姜科）根茎的甲醇提取物及各萃取部位（正己烷、乙酸乙酯和水）对 6 种人癌细胞系（KB、MCF7、A549、Caski、HCT116 和 HT29）的细胞毒活性。乙酸乙酯提取物对 KB、MCF7 和 Caski 细胞具有较高的抑制活性（IC₅₀ 值分别为 9.4、19.7 和 19.8 µg/mL）。从活性乙酸乙酯提取物中分离得到的 flavokawin B（1）、5,6-去氢卡瓦碱（2）、pinostrobin 查尔酮（3）和阿魏酸（4）也进行了细胞毒性评价。其中，pinostrobin 查尔酮（3）和阿魏酸（4）是首次从该植物中分离得到。pinostrobin 查尔酮（3）对所测试的人癌细胞（如 KB、MCF7 和 Caski 细胞）表现出非常显著的细胞毒性活性（IC₅₀ 值分别为 6.2、7.3 和 7.7 µg/mL）。这是益智具有细胞毒性活性的首次报道。
