Ghelishli Noora, Ghasemi Arash, Hosseinimehr Seyed Jalal
Mazandaran University of Medical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Department of Radiopharmacy, Sari, Iran.
Mazandaran University of Medical Sciences, Student Research Committee, Sari, Iran.
Turk J Pharm Sci. 2019 Sep;16(3):366-370. doi: 10.4274/tjps.galenos.2018.50103. Epub 2019 Jul 10.
Ionizing radiation (IR) induces DNA damage in normal cells, leading to genotoxicity. The radioprotective effects of co-treatment with curcumin and piperine were investigated against genotoxicity induced by IR in human normal lymphocytes.
Human blood samples were pretreated with curcumin at different concentrations (5, 10, and 25 μg/mL) and/or piperine (2.5 μg/mL) and then were exposed to IR at a dose 1.5 Gy. The radioprotective effects of curcumin and piperine were assessed by micronucleus (MN) assay.
Curcumin and piperine reduced the percentage of MN induced by IR in lymphocytes. Piperine alone significantly reduced genotoxicity induced by IR as compared to curcumin alone at all concentrations. An additive radioprotective effect was observed with combination of piperine and curcumin at the low concentration of 5 μg/mL, while this synergistic effect was not observed with curcumin at the higher concentrations of 10 and 25 μg/mL.
Piperine has a potent radioprotective effect at low concentration as compare to curcumin. However, an additive radioprotective effect was observed with co-treatment with piperine and curcumin at low concentration, while piperine increased the percentage of MN in normal lymphocytes when co-treated with curcumin at higher concentration.
电离辐射（IR）可诱导正常细胞中的DNA损伤，导致遗传毒性。研究了姜黄素和胡椒碱联合处理对IR诱导的人正常淋巴细胞遗传毒性的辐射防护作用。
将人血样本用不同浓度（5、10和25μg/mL）的姜黄素和/或胡椒碱（2.5μg/mL）预处理，然后以1.5 Gy的剂量暴露于IR。通过微核（MN）试验评估姜黄素和胡椒碱的辐射防护作用。
姜黄素和胡椒碱降低了IR诱导的淋巴细胞中MN的百分比。在所有浓度下，单独使用胡椒碱比单独使用姜黄素能更显著地降低IR诱导的遗传毒性。在低浓度5μg/mL时，观察到胡椒碱和姜黄素联合使用具有相加的辐射防护作用，而在较高浓度10和25μg/mL时，姜黄素未观察到这种协同作用。
与姜黄素相比，胡椒碱在低浓度时具有较强的辐射防护作用。然而，低浓度时胡椒碱和姜黄素联合处理具有相加的辐射防护作用，而高浓度时胡椒碱与姜黄素联合处理会增加正常淋巴细胞中MN的百分比。
