Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, 85-796 Bydgoszcz, Poland.
Genes (Basel). 2020 May 21;11(5):579. doi: 10.3390/genes11050579.
DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters. The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota. In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics. The present study aims to determine the effect of synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens. In ovo stimulation was performed on day 12 of egg incubation. Bioactive compounds delivered in ovo included (S1)- with GOS and (S2)- with RFO. Samples were collected from six individuals from each group on day 42 post-hatching. DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method. Significant changes were observed in DNA methylation of genes in liver including and , after S2 delivery. The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
DNA 甲基化通过向 CpG 岛中基因启动子的半胱氨酸添加甲基残基来抑制 DNA 转录。DNA 甲基化的过程可以被环境因素如肠道微生物群所调节。在禽类中，肠道微生物群的组成可以通过在胚胎期内给予合生素来刺激。本研究旨在确定在胚胎期内给予合生素对肉鸡肝脏 DNA 甲基化水平的影响。在鸡蛋孵化的第 12 天进行胚胎期刺激。在胚胎期内给予的生物活性化合物包括（S1）-与 GOS 和（S2）-与 RFO。在孵化后第 42 天，从每组的六个人中收集样本。使用 qMSP 方法分析基于转录组数据选择的五个基因的 DNA 甲基化。在 S2 给药后，观察到肝脏中包括和在内的基因的 DNA 甲基化发生了显著变化。所得结果证实，胚胎期刺激介导的肝脏代谢基因表达下调具有表观遗传特征。
